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Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia

Title
Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia
Authors
Joo Y.-C.Seo E.-S.Kim Y.-S.Kim K.-R.Park J.-B.Oh D.-K.
Ewha Authors
박진병
SCOPUS Author ID
박진병scopus
Issue Date
2012
Journal Title
Journal of Biotechnology
ISSN
0168-1656JCR Link
Citation
vol. 158, no. 41276, pp. 17 - 23
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A putative fatty acid hydratase from Stenotrophomonas maltophilia was cloned and expressed in Escherichia coli. The recombinant enzyme showed the highest hydration activity for oleic acid among the fatty acids tested, indicating that the enzyme is an oleate hydratase. The optimal conditions for the production of 10-hydroxystearic acid from oleic acid using whole cells of recombinant E. coli containing the oleate hydratase were pH 6.5, 35°C, 0.05% (w/v) Tween 40, 10gl -1 cells, and 50gl -1 oleic acid. Under these conditions, whole recombinant cells produced 49gl -1 10-hydroxystearic acid for 4h, with a conversion yield of 98% (w/w), a volumetric productivity of 12.3gl -1h -1, and a specific productivity of 1.23gg-cells -1h -1, which were 18%, 2.5-, and 2.5-fold higher than those of whole wild-type S. maltophilia cells, respectively. This is the first report of 10-hydroxystearic acid production using recombinant cells and the concentration and productivity are the highest reported thus far among cells. © 2012.
DOI
10.1016/j.jbiotec.2012.01.002
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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