Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황은숙 | * |
dc.date.accessioned | 2016-08-28T12:08:58Z | - |
dc.date.available | 2016-08-28T12:08:58Z | - |
dc.date.issued | 2012 | * |
dc.identifier.issn | 0022-1767 | * |
dc.identifier.other | OAK-8489 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/222383 | - |
dc.description.abstract | IL-10 is a multifunctional cytokine that plays a crucial role in immunity and tolerance. IL-10 is produced by diverse immune cell types, including B cells and subsets of T cells. Although Th1 produce IL-10, their expression levels are much lower than Th2 cells under conventional stimulation conditions. The potential role of E26 transformation-specific 1 (Ets-1) transcription factor as a negative regulator for Il10 gene expression in CD4 + T cells has been implicated previously. In this study, we investigated the underlying mechanism of Ets-1-mediated Il10 gene repression in Th1 cells. Compared with wild type Th1 cells, Ets-1 knockout Th1 cells expressed a significantly higher level of IL-10, which is comparable with that of wild type Th2 cells. Upregulation of IL-10 expression in Ets-1 knockout Th1 cells was accompanied by enhanced chromatin accessibility and increased recruitment of histone H3 acetylation at the Il10 regulatory regions. Reciprocally, Ets-1 deficiency significantly decreased histone deacetylase 1 (HDAC1) enrichment at the Il10 regulatory regions. Treatment with trichostatin A, an inhibitor of HDAC family, significantly increased Il10 gene expression by increasing histone H3 acetylation recruitment. We further demonstrated a physical interaction between Ets-1 and HDAC1. Coexpression of Ets-1 with HDAC1 synergistically repressed IL-10 transcription activity. In summary, our data suggest that an interaction of Ets-1 with HDAC1 represses the Il10 gene expression in Th1 cells. Copyright © 2012 by The American Association of Immunologists, Inc. | * |
dc.language | English | * |
dc.title | Interaction of Ets-1 with HDAC1 represses IL-10 expression in Th1 cells | * |
dc.type | Article | * |
dc.relation.issue | 5 | * |
dc.relation.volume | 188 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2244 | * |
dc.relation.lastpage | 2253 | * |
dc.relation.journaltitle | Journal of Immunology | * |
dc.identifier.doi | 10.4049/jimmunol.1101614 | * |
dc.identifier.wosid | WOS:000300610800025 | * |
dc.identifier.scopusid | 2-s2.0-84863184174 | * |
dc.author.google | Lee C.-G. | * |
dc.author.google | Kwon H.-K. | * |
dc.author.google | Sahoo A. | * |
dc.author.google | Hwang W. | * |
dc.author.google | So J.-S. | * |
dc.author.google | Hwang J.-S. | * |
dc.author.google | Chae C.-S. | * |
dc.author.google | Kim G.-C. | * |
dc.author.google | Kim J.-E. | * |
dc.author.google | So H.-S. | * |
dc.author.google | Hwang E.S. | * |
dc.author.google | Grenningloh R. | * |
dc.author.google | Ho I.-C. | * |
dc.author.google | Im S.-H. | * |
dc.contributor.scopusid | 황은숙(8688011100) | * |
dc.date.modifydate | 20240123102458 | * |