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Intracellular protein delivery by hollow mesoporous silica capsules with a large surface hole

Title
Intracellular protein delivery by hollow mesoporous silica capsules with a large surface hole
Authors
Lim J.-S.Lee K.Choi J.-N.Hwang Y.-K.Yun M.-Y.Kim H.-J.Won Y.S.Kim S.-J.Kwon H.Huh S.
Ewha Authors
김성진
SCOPUS Author ID
김성진scopus
Issue Date
2012
Journal Title
Nanotechnology
ISSN
0957-4484JCR Link
Citation
vol. 23, no. 8
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We prepared cell membrane-permeable hollow mesoporous silica capsules (HMSCs) by a simple new method. CTAB micellar assembly in cholesterol emulsion gave rise to a novel capsular morphology of the HMSC particles. The HMSCs consisted of mesostructured silica walls with a large surface hole (2550nm) and the average particle dimension was 100300nm. They exhibited high surface areas of up to 719.3m 2g 1 and a mesoporous range of pores of 2.42.7nm. The surface-functionalized HMSCs could also be prepared by a similar co-condensation method using tetraethoxysilane with various organoalkoxysilane precursors in the presence of cholesterol. These organically modified HMSCs could be further modified on demand. For example, a carboxy-functionalized HMSC could be surface-functionalized by a green fluorescent 5-aminofluorescein (AFL) through an amidation reaction to afford a fluorescent AFLHMSC. The hollow capsular morphology of the HMSCs with a large surface hole enabled us to develop very efficient intracellular delivery systems for membrane-impermeable ions, molecules, and various functional proteins. Non-covalent sequestration and delivery of proteins as well as covalent linkage of fluorescent molecules on the silica surface are effective for this system. The highly negatively charged green fluorescent probe mag-fluo-4 could be intracellularly delivered into HeLa cells by HMSC without any difficulty. The HMSCs could also effectively transport large functional proteins such as antibodies into HeLa cells. The efficiency of protein delivery by HMSC seems to be 322-fold higher than that of mesoporous silica nanospheres (MSNs) based on confocal laser scanning microscopy (CLSM) analysis. © 2012 IOP Publishing Ltd.
DOI
10.1088/0957-4484/23/8/085101
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자연과학대학 > 화학·나노과학전공 > Journal papers
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