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Hypertonic saline downregulate the production level of lipopolysaccharide- induced migration inhibitory factor in THP-1 cells

Title
Hypertonic saline downregulate the production level of lipopolysaccharide- induced migration inhibitory factor in THP-1 cells
Authors
Han C.Choi S.-H.Yoon Y.-H.Cho Y.-D.Kim J.-Y.Hong Y.-S.Lee S.-W.Moon S.-W.Cho H.-J.Cheon Y.-J.
Ewha Authors
전영진한철
SCOPUS Author ID
전영진scopus; 한철scopus
Issue Date
2012
Journal Title
Journal of the Korean Surgical Society
ISSN
1226-0053JCR Link
Citation
vol. 82, no. 1, pp. 1 - 7
Indexed
SCOPUS WOS scopus
Abstract
Purpose: Macrophage migration inhibitory factor (MIF) may serve as a general marker for systemic inflammation in septic and nonseptic acute critical illness. Additionally, our previous experiment has demonstrated that immunosuppressant Prostaglandin E2 (PGE2) lowered MIF levels and inhibited T-cells proliferation when compared to control levels. The addition of hypertonic saline (HTS) increased MIF production as compared with PGE2-stimulated T-cells in concordance with restore PGE2-suppressed T-cells proliferation. Generally, HTS has been well known for its anti-inflammatory effect so far. Therefore, the experiments were conducted to evaluate MIF after stimulating lipopolysaccharide (LPS) either in the presence or absence of HTS in monocyte, in response to early phase injury. Methods: Human acute monocytic leukemic cell line (THP-1) cells were cultured in RPMI media, to a final concentration of 1 × 10 6 cells/mL. The effect of HTS on LPS-induced MIF was evaluated in monocyte with 1 μg/mL LPS. HTS at 10, 20 or 40 mmol/L above isotonicity was added. MIF concentrations of the supernatant were determined by enzyme-linked immunosorbent assay, and cell lysates were used for Western blots analysis to determine the MIF expression. Results: MIF concentrations in the cell supernatant increased in LPS-induced cells compared to control cells. Also, levels of MIF protein expression were higher in LPS stimulating cells. However, the addition of HTS to LPS stimulated cell restored MIF concentrations and MIF expression. Conclusion: The role of HTS in maintaining physiological balance in human beings, at least in part, should be mediated through the MIF pathway. Copyright © 2012, the Korean Surgical Society.
DOI
10.4174/jkss.2012.82.1.1
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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