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An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells
- An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells
- Kim Y.-H.; Sung H.J.; Kim S.; Kim E.-O.; Lee J.W.; Moon J.Y.; Choi K.; Jung J.-E.; Lee Y.; Koh S.S.; Rhee S.G.; Heo K.; Kim I.-H.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- Cancer Letters
- vol. 313, no. 1, pp. 76 - 83
- SCI; SCIE; SCOPUS
- Previously, we reported that a novel secretory protein, pancreatic adenocarcinoma up-regulated factor (PAUF), which is highly expressed in pancreatic cancer and mediates the growth and metastasis of pancreatic cancer cells. In this study, we generated and characterized a 2'-fluoropyrimidine modified RNA aptamer (P12FR2) directed against human PAUF. P12FR2 binds specifically to human PAUF with an estimated apparent K D of 77. nM. P12FR2 aptamer inhibits PAUF-induced migration of PANC-1, human pancreatic cancer cells, in a wound healing assay. Moreover, intraperitoneal injection of P12FR2 decreased tumor growth by about 60% in an in vivo xenograft model with CFPAC-1 pancreatic cancer cells, without causing a loss of weight in the treated mice. Taken together, we propose here that PAUF-specific RNA aptamer, P12FR2, has the potential to be effective in the therapy of human pancreatic cancer. © 2011 Elsevier Ireland Ltd.
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