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An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells

Title
An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells
Authors
Kim Y.-H.Sung H.J.Kim S.Kim E.-O.Lee J.W.Moon J.Y.Choi K.Jung J.-E.Lee Y.Koh S.S.Rhee S.G.Heo K.Kim I.-H.
Ewha Authors
이서구
SCOPUS Author ID
이서구scopusscopus
Issue Date
2011
Journal Title
Cancer Letters
ISSN
0304-3835JCR Link
Citation
vol. 313, no. 1, pp. 76 - 83
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Previously, we reported that a novel secretory protein, pancreatic adenocarcinoma up-regulated factor (PAUF), which is highly expressed in pancreatic cancer and mediates the growth and metastasis of pancreatic cancer cells. In this study, we generated and characterized a 2'-fluoropyrimidine modified RNA aptamer (P12FR2) directed against human PAUF. P12FR2 binds specifically to human PAUF with an estimated apparent K D of 77. nM. P12FR2 aptamer inhibits PAUF-induced migration of PANC-1, human pancreatic cancer cells, in a wound healing assay. Moreover, intraperitoneal injection of P12FR2 decreased tumor growth by about 60% in an in vivo xenograft model with CFPAC-1 pancreatic cancer cells, without causing a loss of weight in the treated mice. Taken together, we propose here that PAUF-specific RNA aptamer, P12FR2, has the potential to be effective in the therapy of human pancreatic cancer. © 2011 Elsevier Ireland Ltd.
DOI
10.1016/j.canlet.2011.08.027
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일반대학원 > 생명·약학부 > Journal papers
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