Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 전영진 | - |
dc.contributor.author | 한철 | - |
dc.date.accessioned | 2016-08-28T12:08:19Z | - |
dc.date.available | 2016-08-28T12:08:19Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1226-0053 | - |
dc.identifier.other | OAK-8016 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221987 | - |
dc.description.abstract | Purpose: Trauma-induced suppression of cellular immune function likely contributes to sepsis, multiple organ dysfunction syndrome and death. T cell proliferation decreases after traumatic stress. The addition of prostaglandin E2 (PGE 2), which depresses immune function after hemorrhage and trauma, to T-cells decreases T-cell proliferation; and hypertonic saline restores PGE 2-induced T-cell suppression. Recently, it has become apparent that macrophage migration inhibitory factor (MIF) plays a central role in several immune responses, including T-cell proliferation. However, the role of MIF in mediating hypertonic saline (HTS) restoration of T cell dysfunction is unknown. Therefore, we hypothesize that T cell immune restoration by HTS occurs, at least in part, by a MIF-mediated mechanism. Methods: Jurkat cells were cultured in Roswell Park Memorial Institute media, at a final concentration of 2.5 × 10 6 cell/mL. The effects of HTS on T-cell proliferation following PGE 2-induced suppression were evaluated in Jurkat cells: HTS at 20 or 40 mmol/L above isotonicity was added. MIF levels were determined by enzyme-linked immunosorbent assay and western blot analysis. Results: PGE 2 caused a 15.0% inhibition of Jurkat cell proliferation, as compared to the control. MIF levels decreased in PGE 2-suppressed cells, as compared to the control. MIF levels were higher in cells treated with HTS than PGE 2-stimulated cells. Conclusion: The role of HTS in restoring Jurkat cells proliferation suppressed by PGE 2, at least in part, should be mediated through a MIF pathway. Copyright © 2011, the Korean Surgical Society. | - |
dc.language | English | - |
dc.title | Effect of hypertonic saline and macrophage migration inhibitory factor in restoration of T cell dysfunction | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 81 | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 229 | - |
dc.relation.lastpage | 234 | - |
dc.relation.journaltitle | Journal of the Korean Surgical Society | - |
dc.identifier.doi | 10.4174/jkss.2011.81.4.229 | - |
dc.identifier.wosid | WOS:000295424000001 | - |
dc.identifier.scopusid | 2-s2.0-81055137614 | - |
dc.author.google | Yoon Y.-H. | - |
dc.author.google | Choi S.-H. | - |
dc.author.google | Hong Y.-S. | - |
dc.author.google | Lee S.-W. | - |
dc.author.google | Moon S.-W. | - |
dc.author.google | Cho H.-J. | - |
dc.author.google | Han C. | - |
dc.author.google | Cheon Y.-J. | - |
dc.author.google | Bansal V. | - |
dc.contributor.scopusid | 전영진(7003693397) | - |
dc.contributor.scopusid | 한철(56179000200) | - |
dc.date.modifydate | 20200301081000 | - |