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Effect of hypertonic saline and macrophage migration inhibitory factor in restoration of T cell dysfunction

Title
Effect of hypertonic saline and macrophage migration inhibitory factor in restoration of T cell dysfunction
Authors
Yoon Y.-H.Choi S.-H.Hong Y.-S.Lee S.-W.Moon S.-W.Cho H.-J.Han C.Cheon Y.-J.Bansal V.
Ewha Authors
전영진한철
SCOPUS Author ID
전영진scopus; 한철scopus
Issue Date
2011
Journal Title
Journal of the Korean Surgical Society
ISSN
1226-0053JCR Link
Citation
vol. 81, no. 4, pp. 229 - 234
Indexed
SCOPUS WOS scopus
Abstract
Purpose: Trauma-induced suppression of cellular immune function likely contributes to sepsis, multiple organ dysfunction syndrome and death. T cell proliferation decreases after traumatic stress. The addition of prostaglandin E2 (PGE 2), which depresses immune function after hemorrhage and trauma, to T-cells decreases T-cell proliferation; and hypertonic saline restores PGE 2-induced T-cell suppression. Recently, it has become apparent that macrophage migration inhibitory factor (MIF) plays a central role in several immune responses, including T-cell proliferation. However, the role of MIF in mediating hypertonic saline (HTS) restoration of T cell dysfunction is unknown. Therefore, we hypothesize that T cell immune restoration by HTS occurs, at least in part, by a MIF-mediated mechanism. Methods: Jurkat cells were cultured in Roswell Park Memorial Institute media, at a final concentration of 2.5 × 10 6 cell/mL. The effects of HTS on T-cell proliferation following PGE 2-induced suppression were evaluated in Jurkat cells: HTS at 20 or 40 mmol/L above isotonicity was added. MIF levels were determined by enzyme-linked immunosorbent assay and western blot analysis. Results: PGE 2 caused a 15.0% inhibition of Jurkat cell proliferation, as compared to the control. MIF levels decreased in PGE 2-suppressed cells, as compared to the control. MIF levels were higher in cells treated with HTS than PGE 2-stimulated cells. Conclusion: The role of HTS in restoring Jurkat cells proliferation suppressed by PGE 2, at least in part, should be mediated through a MIF pathway. Copyright © 2011, the Korean Surgical Society.
DOI
10.4174/jkss.2011.81.4.229
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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