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Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea

Title
Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea
Authors
Jang J.-E.Lee J.-B.Kim K.-H.Park S.M.Shim B.-S.Cheon I.S.Song M.K.Chang J.
Ewha Authors
김경효장준
SCOPUS Author ID
김경효scopus; 장준scopus
Issue Date
2011
Journal Title
PLoS ONE
ISSN
1932-6203JCR Link
Citation
vol. 6, no. 9
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Human respiratory syncytial virus (HRSV) is a significant cause of upper and lower respiratory tract illness mainly in infants and young children worldwide. HRSV is divided into two subgroups, HRSV-A and HRSV-B, based on sequence variation within the G gene. Despite its importance as a respiratory pathogen, there is currently no safe and effective vaccine for HRSV. In this study, we have detected and identified the HRSV by RT-PCR from nasopharyngeal aspirates of Korean pediatric patients. Interestingly, all HRSV-B isolates exhibited unique deletion of 6 nucleotides and duplication of 60 nucleotides in the G gene. We successfully amplified two isolates ('KR/A/09-8' belonging to HRSV-A and 'KR/B/10-12' to HRSV-B) on large-scale, and evaluated the cross-protective efficacy of our recombinant adenovirus-based HRSV vaccine candidate, rAd/3xG, by challenging the immunized mice with these isolates. The single intranasal immunization with rAd/3xG protected the mice completely from KR/A/09-8 infection and partially from KR/B/10-12 infection. Our study contributes to the understanding of the genetic characteristics and distribution of subgroups in the seasonal HRSV epidemics in Korea and, for the first time, to the evaluation of the cross-protective efficacy of RSV vaccine against HRSV-A and -B field-isolates. © 2011 Jang et al.
DOI
10.1371/journal.pone.0023797
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의학전문대학원 > 의학과 > Journal papers
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