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Base pair opening kinetics study of the aegPNA:DNA hydrid duplex containing a site-specific GNA-like chiral PNA monomer

Title
Base pair opening kinetics study of the aegPNA:DNA hydrid duplex containing a site-specific GNA-like chiral PNA monomer
Authors
Seo Y.-J.Lim J.Lee E.-H.Ok T.Yoon J.Lee J.-H.Lee H.-S.
Ewha Authors
윤주영
SCOPUS Author ID
윤주영scopus
Issue Date
2011
Journal Title
Nucleic Acids Research
ISSN
0305-1048JCR Link
Citation
vol. 39, no. 16, pp. 7329 - 7335
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Peptide nucleic acids (PNA) are one of the most widely used synthetic DNA mimics where the four bases are attached to a N-(2-aminoethyl)glycine (aeg) backbone instead of the negative-charged phosphate backbone in DNA. We have developed a chimeric PNA (chiPNA), in which a chiral GNA-like γ 3T monomer is incorporated into aegPNA backbone. The base pair opening kinetics of the aegPNA:DNA and chiPNA:DNA hybrid duplexes were studied by NMR hydrogen exchange experiments. This study revealed that the aegPNA:DNA hybrid is much more stable duplex and is less dynamic compared to DNA duplex, meaning that base pairs are opened and reclosed much more slowly. The site-specific incorporation of γ 3T monomer in the aegPNA:DNA hybrid can destabilize a specific base pair and its neighbors, maintaining the thermal stabilities and dynamic properties of other base pairs. Our hydrogen exchange study firstly revealed the unique kinetic features of base pairs in the aegPNA:DNA and chiPNA:DNA hybrids, which will provide an insight into the development of methodology for specific DNA recognition using PNA fragments. © 2011 The Author(s).
DOI
10.1093/nar/gkr360
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자연과학대학 > 화학·나노과학전공 > Journal papers
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