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The THO complex is required for nucleolar integrity in Drosophila spermatocytes

Title
The THO complex is required for nucleolar integrity in Drosophila spermatocytes
Authors
Moon S.Cho B.Min S.-H.Lee D.Chung Y.D.
Ewha Authors
이대기
SCOPUS Author ID
이대기scopus
Issue Date
2011
Journal Title
Development
ISSN
0950-1991JCR Link
Citation
vol. 138, no. 17, pp. 3835 - 3845
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The THO complex is a conserved multisubunit protein complex that functions in the formation of export-competent messenger ribonucleoprotein (mRNP). Although the complex has been studied extensively at the single-cell level, its exact role at the multicellular organism level has been poorly understood. Here, we isolated a novel Drosophila male sterile mutant, garmcho (garm). Positional cloning indicated that garm encodes a subunit of the Drosophila THO complex, THOC5. Flies lacking THOC5 showed a meiotic arrest phenotype with severe nucleolar disruption in primary spermatocytes. A functional GFP-tagged fusion protein, THOC5-GFP, revealed a unique pattern of THOC5 localization near the nucleolus. The nucleolar distribution of a testisspecific TATA binding protein (TBP)-associated factor (tTAF), SA, which is required for the expression of genes responsible for sperm differentiation, was severely disrupted in mutant testes lacking THOC5. But THOC5 appeared to be largely dispensable for the expression and nuclear export of either tTAF target mRNAs or tTAF-independent mRNAs. Taken together, our study suggests that the Drosophila THO complex is necessary for proper spermatogenesis by contribution to the establishment or maintenance of nucleolar integrity rather than by nuclear mRNA export in spermatocytes. © 2011. Published by The Company of Biologists Ltd.
DOI
10.1242/dev.056945
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자연과학대학 > 생명과학전공 > Journal papers
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