View : 13 Download: 2

Systemic analysis of Heat Shock response induced by Heat Shock and a Proteasome inhibitor MG132

Title
Systemic analysis of Heat Shock response induced by Heat Shock and a Proteasome inhibitor MG132
Authors
Kim H.-J.Joo H.J.Kim Y.H.Ahn S.Chang J.Hwang K.-B.Lee D.-H.Lee K.-J.
Ewha Authors
이동희이공주장준김희정
SCOPUS Author ID
이동희scopus; 이공주scopus; 장준scopus; 김희정scopusscopusscopus
Issue Date
2011
Journal Title
PLoS ONE
ISSN
1932-6203JCR Link
Citation
vol. 6, no. 6
Indexed
SCIE; SCOPUS WOS scopus
Abstract
The molecular basis of heat shock response (HSR), a cellular defense mechanism against various stresses, is not well understood. In this, the first comprehensive analysis of gene expression changes in response to heat shock and MG132 (a proteasome inhibitor), both of which are known to induce heat shock proteins (Hsps), we compared the responses of normal mouse fibrosarcoma cell line, RIF- 1, and its thermotolerant variant cell line, TR-RIF-1 (TR), to the two stresses. The cellular responses we examined included Hsp expressions, cell viability, total protein synthesis patterns, and accumulation of poly-ubiquitinated proteins. We also compared the mRNA expression profiles and kinetics, in the two cell lines exposed to the two stresses, using microarray analysis. In contrast to RIF-1 cells, TR cells resist heat shock caused changes in cell viability and whole-cell protein synthesis. The patterns of total cellular protein synthesis and accumulation of poly-ubiquitinated proteins in the two cell lines were distinct, depending on the stress and the cell line. Microarray analysis revealed that the gene expression pattern of TR cells was faster and more transient than that of RIF-1 cells, in response to heat shock, while both RIF-1 and TR cells showed similar kinetics of mRNA expression in response to MG132. We also found that 2,208 genes were up-regulated more than 2 fold and could sort them into three groups: 1) genes regulated by both heat shock and MG132, (e.g. chaperones); 2) those regulated only by heat shock (e.g. DNA binding proteins including histones); and 3) those regulated only by MG132 (e.g. innate immunity and defense related molecules). This study shows that heat shock and MG132 share some aspects of HSR signaling pathway, at the same time, inducing distinct stress response signaling pathways, triggered by distinct abnormal proteins. © 2011 Kim et al.
DOI
10.1371/journal.pone.0020252
Appears in Collections:
일반대학원 > 바이오융합과학과 > Journal papers
Files in This Item:
001.pdf(2.25 MB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE