Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 조한중 | - |
dc.date.accessioned | 2016-08-28T12:08:50Z | - |
dc.date.available | 2016-08-28T12:08:50Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.other | OAK-7694 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221716 | - |
dc.description.abstract | Background: Aortic valve (AV) calcification preferentially occurs on the fibrosa side while the ventricularis side remains relatively unaffected. Here, we tested the hypothesis that side-dependent activation of bone morphogenic protein (BMP) pathway in the endothelium of the ventricularis and fibrosa is associated with human AV calcification. Methods and Results: Human calcified AVs obtained from AV replacement surgeries and non-calcified AVs from heart transplantations were used for immunohistochemical studies. We found SMAD-1/5/8 phosphorylation (a canonical BMP pathway) was higher in the calcified fibrosa than the non-calcified fibrosa while SMAD-2/3 phosphorylation (a canonical TGFβ pathway) did not show any difference. Interestingly, we found that BMP-2/4/6 expression was significantly higher on the ventricularis endothelium compared to the fibrosa in both calcified and non-calcified AV cusps; however, BMP antagonists (crossvienless-2/BMPER and noggin) expression was significantly higher on the ventricularis endothelium compared to the fibrosa in both disease states. Moreover, significant expression of inhibitory SMAD-6 expression was found only in the non-calcified ventricularis endothelium. Conclusions: SMAD-1/5/8 is preferentially activated in the calcified fibrosa endothelium of human AVs and it correlates with low expression of BMP antagonists and inhibitory SMAD6. These results suggest a dominant role of BMP antagonists in the side-dependent calcification of human AVs. © 2011 Ankeny et al. | - |
dc.language | English | - |
dc.title | Preferential activation of SMAD1/5/8 on the fibrosa endothelium in calcified human aortic valves - association with low BMP antagonists and SMAD6 | - |
dc.type | Article | - |
dc.relation.issue | 6 | - |
dc.relation.volume | 6 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.journaltitle | PLoS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0020969 | - |
dc.identifier.wosid | WOS:000291730000033 | - |
dc.identifier.scopusid | 2-s2.0-79958818345 | - |
dc.author.google | Ankeny R.F. | - |
dc.author.google | Thourani V.H. | - |
dc.author.google | Weiss D. | - |
dc.author.google | Vega J.D. | - |
dc.author.google | Taylor W.R. | - |
dc.author.google | Nerem R.M. | - |
dc.author.google | Jo H. | - |
dc.date.modifydate | 20160429000000 | - |