Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박혜경 | * |
dc.contributor.author | 이지희 | * |
dc.contributor.author | 정영해 | * |
dc.date.accessioned | 2016-08-28T12:08:49Z | - |
dc.date.available | 2016-08-28T12:08:49Z | - |
dc.date.issued | 2011 | * |
dc.identifier.issn | 0014-4886 | * |
dc.identifier.other | OAK-7678 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221705 | - |
dc.description.abstract | Human immunodeficiency virus-1 (HIV-1) associated dementia (HAD) has been attributed to an encephalitis resulting from intense infiltration of monocytes. Evidence suggests that the viral protein Tat, which is released actively from HIV-1 infected cells, can contribute significantly to this process. Therefore, the principal objective of this study was to evaluate the potential molecular basis for the role of extracellular HIV-1 Tat in the induction of monocyte chemotactic protein-1 (MCP-1/CCL2) in the hippocampus, which is primarily linked to cognitive function and most commonly damaged in HAD. We also attempted to identify the mechanism by which resveratrol (trans-3,5,4'-trihydroxystilbene) modulates MCP-1 release in hippocampal tissues exposed to Tat. An ex vivo study using rat hippocampal slices demonstrated a time- and dose-dependent increase in MCP-1 production from Tat-treated hippocampal tissues. This increase was accompanied by the activation of the MEK/ERK pathway and TNF-α production. Tat-induced MCP-1 release was abrogated by inhibitors of tyrosine kinases (TK), herbimycin A or genistein, a finding that supports the MAPK signaling mechanism. The inhibition of the ERK1/2 pathway with SL327 induced a near-complete abolition of the observed Tat-induced effects. Furthermore, anti-TNF-α antibodies suppressed Tat-induced MCP-1 release. Resveratrol, to a level similar to that of SL327, downregulated Tat-induced proinflammatory responses via the inactivation of ERK1/2. These results indicate that the activation of the ERK1/2 pathway and TK are critical factors in the production of TNF-α and MCP-1 in the Tat-exposed hippocampus. Additionally, the inhibition of Tat-induced production of MCP-1 and TNF-α via the inactivation of the ERK1/2 pathway may represent the anti-inflammatory mechanism of resveratrol in the hippocampus. © 2011 Elsevier Inc. | * |
dc.language | English | * |
dc.title | Extracellular HIV-1 Tat upregulates TNF-α dependent MCP-1/CCL2 production via activation of ERK1/2 pathway in rat hippocampal slice cultures: Inhibition by resveratrol, a polyphenolic phytostilbene | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 229 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 399 | * |
dc.relation.lastpage | 408 | * |
dc.relation.journaltitle | Experimental Neurology | * |
dc.identifier.doi | 10.1016/j.expneurol.2011.03.006 | * |
dc.identifier.wosid | WOS:000291419400025 | * |
dc.identifier.scopusid | 2-s2.0-79956097572 | * |
dc.author.google | Lee E.O. | * |
dc.author.google | Kim S.E. | * |
dc.author.google | Park H.K. | * |
dc.author.google | Kang J.L. | * |
dc.author.google | Chong Y.H. | * |
dc.contributor.scopusid | 박혜경(36014150800) | * |
dc.contributor.scopusid | 이지희(7404517577) | * |
dc.contributor.scopusid | 정영해(7201371824) | * |
dc.date.modifydate | 20240423081003 | * |