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SiRNA targeting livin decreases tumor in a xenograft model

Title
SiRNA targeting livin decreases tumor in a xenograft model
Authors
Cancer F.C.Oh B.-Y.Lee R.-A.Kim K.H.
Ewha Authors
김광호이령아
SCOPUS Author ID
김광호scopus; 이령아scopus
Issue Date
2011
Journal Title
World Journal of Gastroenterology
ISSN
1007-9327JCR Link
Citation
vol. 17, no. 20, pp. 2563 - 2571
Indexed
SCIE; SCOPUS WOS scopus
Abstract
AIM: To evaluate the effect of silencing Livin gene expression with siRNA to apoptosis and proliferation in a colon cancer cell line. METHODS: To investigate the anticancer effect of silencing Livin gene expression, we established an siRNA transfected cell line using the HCT116 colon cancer cell line. After confirming the successful transfection, MTT assay, flow cytometry and annexin V staining were employed to evaluate the antiapoptotic effect. To confirm the in vivo effect of Livin-siRNA, different doses of LivinsiRNA were injected into xenografted tumors in BALB/c nude mice model. RESULTS: Livin expression was dramatically decreased after siRNA transfection, especially at 25 μmol/L of siRNA, but this suppression was not dose-dependent. The cell count at 18 h after transfection was significantly reduced as compared with controls (P < 0.01), but tended not to decrease proportionally depending on transfected dose or time. MTT assay revealed that silencing the Livin gene suppressed cellular proliferation at 18 h after transfection (P = 0.04); however, the inhibitory effect disappeared thereafter. Also, there was no significant difference in cellular proliferation depending on siRNA dose. The rate of apoptosis also increased with silencing of the Livin gene. In vivo, the tumor size significantly decreased after LivinsiRNA injection at 20 μmol/L concentration (P = 0.03). There were no significant body weight changes of mice after siRNA injection. Histologic examination revealed no significant toxic reaction in kidney, liver and brain of mice. CONCLUSION: SiRNA-mediated downregulation of Livin expression can induce apoptosis in colon cancer in vitro and in vivo, which suggests the possibility of new cancer therapeutics using siRNA. © 2011 Baishideng.
DOI
10.3748/wjg.v17.i20.2563
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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