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Local kallikrein-kinin system is involved in podocyte apoptosis under diabetic conditions

Title
Local kallikrein-kinin system is involved in podocyte apoptosis under diabetic conditions
Authors
Kwak S.-J.Paeng J.Kim D.H.Lee S.H.Nam B.-Y.Kang H.Y.Li J.J.Jung D.-S.Han S.H.Ryu D.-R.Park J.T.Chang T.I.Yoo T.-H.Han D.S.Kang S.-W.
Ewha Authors
류동열
SCOPUS Author ID
류동열scopus
Issue Date
2011
Journal Title
Apoptosis
ISSN
1360-8185JCR Link
Citation
vol. 16, no. 5, pp. 478 - 490
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The kallikrein-kinin system (KKS) serves as the physiologic counterbalance to the renin-angiotensin system. This study was conducted to examine the changes in the expression of KKS components in podocytes under diabetic conditions and to elucidate the functional role of bradykinin (BK) in diabetes-associated podocyte apoptosis. Thirty-two rats were injected with either diluent (n = 16, C) or with streptozotocin intraperitoneally (n = 16, DM), and 8 rats from each group were treated with BK infusion for 6 weeks. Immortalized mouse podocytes were cultured in media containing 5.6 mmol/l glucose (NG), NG + 10 -7 mol/l AII (AII), or 30 mmol/l glucose (HG) with or without 10 -8 mol/l BK. Urinary albumin excretion was significantly higher in DM rats, and this increase was ameliorated by BK. Not only kininogen, kallikrein, and BK B1- and B2-receptor expression but also BK levels were significantly decreased in DM glomeruli and in cultured podocytes exposed to HG. The changes in the expressions of apoptosis-related molecules and the increase in the number of apoptotic cells in DM glomeruli as well as in HG- and AII-stimulated podocytes were significantly abrogated by BK. The suppressed KSS within podocytes under diabetic condition was associated with podocyte apoptosis, suggesting that BK may be beneficial in preventing podocyte loss in diabetic nephropathy. © 2011 Springer Science+Business Media, LLC.
DOI
10.1007/s10495-011-0585-1
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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