Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김희선 | * |
dc.date.accessioned | 2016-08-28T12:08:31Z | - |
dc.date.available | 2016-08-28T12:08:31Z | - |
dc.date.issued | 2011 | * |
dc.identifier.issn | 0304-3940 | * |
dc.identifier.other | OAK-7488 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221552 | - |
dc.description.abstract | Ethyl pyruvate (EP) is a stable derivative of pyruvate and has been identified as a therapeutic agent for various inflammatory diseases. In the present study, we showed that EP and sodium pyruvate (SP) inhibited the production of TNF-α, nitric oxide (NO), or reactive oxygen species (ROS) in LPS-stimulated BV2 microglial cells. The inhibitory effects of EP were more potent than SP. Because matrix metalloproteinase-9 (MMP-9) plays a key role in neuroinflammation, as well as in neuronal cell death, we examined the effect of EP on MMP-9 expression. RT-PCR and Western blot analyses revealed that EP inhibits MMP-9 expression at mRNA and protein levels in LPS-stimulated BV2 cells. In addition, EP suppressed MMP-9 secretion, as demonstrated by gelatin zymography analysis. In contrast, SP did not affect MMP-9 expression at an equivalent concentration of EP. Further mechanistic studies revealed that EP inhibits MMP-9 promoter activity by reducing the binding of NF-κB and AP-1 to its cognitive binding sites. In addition, EP suppressed LPS-induced phosphorylation of p38 MAPK, ERK, and Akt, which are upstream signaling molecules in MMP-9 gene expression. Taken together, our data suggest that the inhibition of MMP-9 may be one of the factors contributing to anti-inflammatory activity of EP in LPS-stimulated microglia. © 2011 Elsevier Ireland Ltd. | * |
dc.language | English | * |
dc.title | Inhibitory mechanism of MMP-9 gene expression by ethyl pyruvate in lipopolysaccharide-stimulated BV2 microglial cells | * |
dc.type | Article | * |
dc.relation.issue | 41276 | * |
dc.relation.volume | 493 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 38 | * |
dc.relation.lastpage | 43 | * |
dc.relation.journaltitle | Neuroscience Letters | * |
dc.identifier.doi | 10.1016/j.neulet.2011.02.016 | * |
dc.identifier.wosid | WOS:000289180800008 | * |
dc.identifier.scopusid | 2-s2.0-79952620639 | * |
dc.author.google | Lee E.-J. | * |
dc.author.google | Kim H.-S. | * |
dc.contributor.scopusid | 김희선(57191372551) | * |
dc.date.modifydate | 20240118140922 | * |