Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이서구 | * |
dc.contributor.author | 배수한 | * |
dc.contributor.author | 길인섭 | * |
dc.contributor.author | 우현애 | * |
dc.date.accessioned | 2016-08-28T12:08:23Z | - |
dc.date.available | 2016-08-28T12:08:23Z | - |
dc.date.issued | 2011 | * |
dc.identifier.issn | 0270-9139 | * |
dc.identifier.other | OAK-7408 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221479 | - |
dc.description.abstract | Peroxiredoxins (Prxs) are peroxidases that catalyze the reduction of reactive oxygen species (ROS). The active site cysteine residue of members of the 2-Cys Prx subgroup (Prx I to IV) of Prxs is hyperoxidized to cysteine sulfinic acid (Cys-SO 2) during catalysis with concomitant loss of peroxidase activity. Reactivation of the hyperoxidized Prx is catalyzed by sulfiredoxin (Srx). Ethanol consumption induces the accumulation of cytochrome P450 2E1 (CYP2E1), a major contributor to ethanol-induced ROS production in the liver. We now show that chronic ethanol feeding markedly increased the expression of Srx in the liver of mice in a largely Nrf2-dependent manner. Among Prx I to IV, only Prx I was found to be hyperoxidized in the liver of ethanol-fed wildtype mice, and the level of Prx I-SO 2 increased to ≈30% to 50% of total Prx I in the liver of ethanol-fed Srx -/- mice. This result suggests that Prx I is the most active 2-Cys Prx in elimination of ROS from the liver of ethanol-fed mice and that, despite the up-regulation of Srx expression by ethanol, the capacity of Srx is not sufficient to counteract the hyperoxidation of Prx I that occurs during ROS reduction. A protease protection assay revealed that a large fraction of Prx I is located together with CYP2E1 at the cytosolic side of the endoplasmic reticulum membrane. The selective role of Prx I in ROS removal is thus likely attributable to the proximity of Prx I and CYP2E1. Conclusion: The pivotal functions of Srx and Prx I in protection of the liver in ethanol-fed mice was evident from the severe oxidative damage observed in mice lacking either Srx or Prx I. © 2010 American Association for the Study of Liver Diseases. | * |
dc.language | English | * |
dc.title | Concerted action of sulfiredoxin and peroxiredoxin I protects against alcohol-induced oxidative injury in mouse liver | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 53 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 945 | * |
dc.relation.lastpage | 953 | * |
dc.relation.journaltitle | Hepatology | * |
dc.identifier.doi | 10.1002/hep.24104 | * |
dc.identifier.wosid | WOS:000288211200025 | * |
dc.identifier.scopusid | 2-s2.0-79952224744 | * |
dc.author.google | Bae S.H. | * |
dc.author.google | Sung S.H. | * |
dc.author.google | Cho E.J. | * |
dc.author.google | Lee S.K. | * |
dc.author.google | Lee H.E. | * |
dc.author.google | Woo H.A. | * |
dc.author.google | Yu D.-Y. | * |
dc.author.google | Kil I.S. | * |
dc.author.google | Rhee S.G. | * |
dc.contributor.scopusid | 이서구(7401852092) | * |
dc.contributor.scopusid | 길인섭(152039) | * |
dc.contributor.scopusid | 우현애(8068619500) | * |
dc.date.modifydate | 20240215164922 | * |