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Concerted action of sulfiredoxin and peroxiredoxin I protects against alcohol-induced oxidative injury in mouse liver

Title
Concerted action of sulfiredoxin and peroxiredoxin I protects against alcohol-induced oxidative injury in mouse liver
Authors
Bae S.H.Sung S.H.Cho E.J.Lee S.K.Lee H.E.Woo H.A.Yu D.-Y.Kil I.S.Rhee S.G.
Ewha Authors
이서구배수한길인섭우현애
SCOPUS Author ID
이서구scopusscopus; 우현애scopus
Issue Date
2011
Journal Title
Hepatology
ISSN
0270-9139JCR Link
Citation
vol. 53, no. 3, pp. 945 - 953
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Peroxiredoxins (Prxs) are peroxidases that catalyze the reduction of reactive oxygen species (ROS). The active site cysteine residue of members of the 2-Cys Prx subgroup (Prx I to IV) of Prxs is hyperoxidized to cysteine sulfinic acid (Cys-SO 2) during catalysis with concomitant loss of peroxidase activity. Reactivation of the hyperoxidized Prx is catalyzed by sulfiredoxin (Srx). Ethanol consumption induces the accumulation of cytochrome P450 2E1 (CYP2E1), a major contributor to ethanol-induced ROS production in the liver. We now show that chronic ethanol feeding markedly increased the expression of Srx in the liver of mice in a largely Nrf2-dependent manner. Among Prx I to IV, only Prx I was found to be hyperoxidized in the liver of ethanol-fed wildtype mice, and the level of Prx I-SO 2 increased to ≈30% to 50% of total Prx I in the liver of ethanol-fed Srx -/- mice. This result suggests that Prx I is the most active 2-Cys Prx in elimination of ROS from the liver of ethanol-fed mice and that, despite the up-regulation of Srx expression by ethanol, the capacity of Srx is not sufficient to counteract the hyperoxidation of Prx I that occurs during ROS reduction. A protease protection assay revealed that a large fraction of Prx I is located together with CYP2E1 at the cytosolic side of the endoplasmic reticulum membrane. The selective role of Prx I in ROS removal is thus likely attributable to the proximity of Prx I and CYP2E1. Conclusion: The pivotal functions of Srx and Prx I in protection of the liver in ethanol-fed mice was evident from the severe oxidative damage observed in mice lacking either Srx or Prx I. © 2010 American Association for the Study of Liver Diseases.
DOI
10.1002/hep.24104
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일반대학원 > 생명·약학부 > Journal papers
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