Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박미혜 | * |
dc.date.accessioned | 2016-08-28T12:08:21Z | - |
dc.date.available | 2016-08-28T12:08:21Z | - |
dc.date.issued | 2011 | * |
dc.identifier.issn | 1046-7408 | * |
dc.identifier.other | OAK-7389 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221461 | - |
dc.description.abstract | Problem Hypoxia is commonly associated with complicated pregnancies such as intrauterine growth restriction. We evaluated the effects of hypoxia on phospho (p)-eNOS, p-ERK, p-AKT and apoptosis in human trophoblast. Method of study Isolated trophoblast were cultured in 21% oxygen or 2% oxygen for 24, 48 and 72hr. p-eNOS, p-ERK and p-AKT protein were assessed by Western blot and apoptosis by TUNEL assay. NOx was determined in the culture media. Results Compared to controls, hypoxia-exposed CT showed the following: (1) decreased eNOS at 48 and 72hr, (2) increased p-eNOS at 48 hr, (3) no differences in total NOx production, (4) increased p-ERK at 24, 48 and 72hr, (5) increased p-AKT at 24hr (P<0.05) and (6) increased apoptosis at 48 hr. Conclusion Hypoxia increases activation of p-ERK and induces apoptosis of cultured trophoblast. Hypoxia decreases overall total eNOS but increases p-eNOS, which may allow for NO production to be maintained in trophoblast cells. © 2010 John Wiley & Sons A/S. | * |
dc.language | English | * |
dc.title | Effect of Hypoxia on Endothelial Nitric Oxide Synthase, NO Production, Intracellular Survival Signaling (p-ERK1/2 and p-AKT) and Apoptosis in Human Term Trophoblast | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 65 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 407 | * |
dc.relation.lastpage | 414 | * |
dc.relation.journaltitle | American Journal of Reproductive Immunology | * |
dc.identifier.doi | 10.1111/j.1600-0897.2010.00886.x | * |
dc.identifier.wosid | WOS:000288025000005 | * |
dc.identifier.scopusid | 2-s2.0-79952295070 | * |
dc.author.google | Park M.-H. | * |
dc.author.google | Galan H.L. | * |
dc.author.google | Arroyo J.A. | * |
dc.contributor.scopusid | 박미혜(57218662303;58492640400) | * |
dc.date.modifydate | 20240118164151 | * |