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Bacterial diversity dynamics in a long-term petroleum-contaminated soil

Title
Bacterial diversity dynamics in a long-term petroleum-contaminated soil
Authors
Lee E.-H.Lee S.H.Cho K.-S.
Ewha Authors
조경숙이은희
SCOPUS Author ID
조경숙scopus; 이은희scopusscopus
Issue Date
2011
Journal Title
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
ISSN
1093-4529JCR Link
Citation
vol. 46, no. 3, pp. 281 - 290
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Bacterial diversity dynamics were investigated in the soil samples in different distances and depths from/at a long-term petroleumcontaminated site. Microbial activity in the soil samples showed ATP values closely correlated with organic matter content (OC) and total petroleum hydrocarbon (TPH). Bacterial community diversity (H) and evenness (J) using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and PCR-T-RFLP (terminal restriction fragment length polymorphism) results showed positive correlation with concentration of TPH or OC, but tmoA (toluene monooxygenase gene)-based bacterial H and J using a PCR-T-RFLP result did not. No significant difference of H and J values in the bacterial and the tmoA communities was observed. The bacterial community structure characterized by PCR-DGGE and PCR-T-RFLP techniques showed similarity according to soil sampling distance rather than soil sampling depth. Canonical correspondence analysis demonstrated that OC including TPH had the most significant effect on the bacterial community diversity at the long-term petroleum-contaminated site. Copyright © Taylor & Francis Group, LLC.
DOI
10.1080/10934529.2011.535435
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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