Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이대기 | * |
dc.date.accessioned | 2016-08-28T12:08:18Z | - |
dc.date.available | 2016-08-28T12:08:18Z | - |
dc.date.issued | 2011 | * |
dc.identifier.issn | 0006-291X | * |
dc.identifier.other | OAK-7354 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221429 | - |
dc.description.abstract | Sustained EGF receptor (EGFR) phosphorylation by de novo synthesis of EGFR ligands plays an essential role in mediating luteinizing hormone (LH)-induced ovulation process in the preovulatory follicles (POFs). In the present study, the effect of epiregulin (EREG) on oocyte maturation and ovulation was investigated using Ereg knockout (Ereg -/-) mice congenic on a C57BL/6 background. Rate of spontaneous oocyte meiotic resumption of denuded oocytes (DOs) or cumulus cell-oocyte complexes (COCs) in vitro is similar between wild-type and Ereg -/- mice. However, gonadotropin-induced meiotic resumption in vivo is attenuated, and the number of COCs with expanded cumulus matrix and superovulated eggs dramatically decrease in Ereg -/- mice. Nonetheless, the number of eggs ovulated during normal estrus cycles and litter sizes in Ereg -/- mice are comparable to those of wild-type littermates. In contrast to other EGFR ligands, induction of amphiregulin (Areg) mRNA is severely reduced in ovaries collected from Ereg -/- mice either after human chorionic gonadotropin (hCG) treatment in immature mice or LH surge in adults. Gonadotropin-induced EGFR and ERBB2 phosphorylation in ovaries is attenuated in immature Ereg -/- mice, and MAPK3/1 phosphorylation and prostaglandin synthase 2 (PTGS2) protein levels are reduced. This attenuation, however, is no longer detectable in adult Ereg -/- mice after LH surge. This study implicates that EREG mediates signals downstream of Areg mRNA expression and that EGFR-ERBB2 signals contributes to regulation of ovulation process. © 2011 Elsevier Inc. | * |
dc.language | English | * |
dc.title | Epiregulin-dependent amphiregulin expression and ERBB2 signaling are involved in luteinizing hormone-induced paracrine signaling pathways in mouse ovary | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 405 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 319 | * |
dc.relation.lastpage | 324 | * |
dc.relation.journaltitle | Biochemical and Biophysical Research Communications | * |
dc.identifier.doi | 10.1016/j.bbrc.2011.01.039 | * |
dc.identifier.wosid | WOS:000287627500030 | * |
dc.identifier.scopusid | 2-s2.0-79651474412 | * |
dc.author.google | Kim K. | * |
dc.author.google | Lee H. | * |
dc.author.google | Threadgill D.W. | * |
dc.author.google | Lee D. | * |
dc.contributor.scopusid | 이대기(37047040400) | * |
dc.date.modifydate | 20231120165418 | * |