Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤여준 | * |
dc.contributor.author | 이상기 | * |
dc.date.accessioned | 2016-08-28T12:08:14Z | - |
dc.date.available | 2016-08-28T12:08:14Z | - |
dc.date.issued | 2011 | * |
dc.identifier.issn | 0002-7863 | * |
dc.identifier.other | OAK-7321 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221399 | - |
dc.description.abstract | The allyl moiety of the immunosuppressive agent FK506 is structurally unique among polyketides and critical for its potent biological activity. Here, we detail the biosynthetic pathway to allylmalonyl-coenzyme A (CoA), from which the FK506 allyl group is derived, based on a comprehensive chemical, biochemical, and genetic interrogation of three FK506 gene clusters. A discrete polyketide synthase (PKS) with noncanonical domain architecture presumably in coordination with the fatty acid synthase pathway of the host catalyzes a multistep enzymatic reaction to allylmalonyl-CoA via frans-2-pentenylacyl carrier protein. Characterization of this discrete pathway facilitated the engineered biosynthesis of novel allyl group-modified FK506 analogues, 36-fluoro-FK520 and 36-methyl-FK506, the latter of which exhibits improved neurite outgrowth activity. This unique feature of FK506 biosynthesis, in which a dedicated PKS provides an atypical extender unit for the main modular PKS, illuminates a new strategy for the combinatorial biosynthesis of designer macrolide scaffolds as well as FK506 analogues. © 2010 American Chemical Society. | * |
dc.language | English | * |
dc.title | Biosynthesis of the allylmalonyl-CoA extender unit for the FK506 polyketide synthase proceeds through a dedicated polyketide synthase and facilitates the mutasynthesis of analogues | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 133 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 976 | * |
dc.relation.lastpage | 985 | * |
dc.relation.journaltitle | Journal of the American Chemical Society | * |
dc.identifier.doi | 10.1021/ja108399b | * |
dc.identifier.wosid | WOS:000287295300060 | * |
dc.identifier.scopusid | 2-s2.0-79851505835 | * |
dc.author.google | Mo S. | * |
dc.author.google | Kim D.H. | * |
dc.author.google | Lee J.H. | * |
dc.author.google | Park J.W. | * |
dc.author.google | Basnet D.B. | * |
dc.author.google | Ban Y.H. | * |
dc.author.google | Yoo Y.J. | * |
dc.author.google | Chen S.-W. | * |
dc.author.google | Park S.R. | * |
dc.author.google | Choi E.A. | * |
dc.author.google | Kim E. | * |
dc.author.google | Jin Y.-Y. | * |
dc.author.google | Lee S.-K. | * |
dc.author.google | Park J.Y. | * |
dc.author.google | Liu Y. | * |
dc.author.google | Lee M.O. | * |
dc.author.google | Lee K.S. | * |
dc.author.google | Kim S.J. | * |
dc.author.google | Kim D. | * |
dc.author.google | Park B.C. | * |
dc.author.google | Lee S.-G. | * |
dc.author.google | Kwon H.J. | * |
dc.author.google | Suh J.-W. | * |
dc.author.google | Moore B.S. | * |
dc.author.google | Lim S.-K. | * |
dc.author.google | Yoon Y.J. | * |
dc.contributor.scopusid | 윤여준(7402126465) | * |
dc.contributor.scopusid | 이상기(15082786300) | * |
dc.date.modifydate | 20240123104103 | * |