Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오억수 | - |
dc.date.accessioned | 2016-08-28T12:08:11Z | - |
dc.date.available | 2016-08-28T12:08:11Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1522-8002 | - |
dc.identifier.other | OAK-7286 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221366 | - |
dc.description.abstract | Increased expression and/or activation of H-Ras are often associated with tumor aggressiveness in breast cancer. Previously, we showed that H-Ras, but not N-Ras, induces MCF10A human breast epithelial cell invasion and migration, whereas both H-Ras and N-Ras induce cell proliferation and phenotypic transformation. In an attempt to determine the sequence requirement directing the divergent phenotype induced by H-Ras and N-Ras with a focus on the induction of human breast cell invasion, we investigated the structural and functional relationships between H-Ras and N-Ras using domain-swap and site-directed mutagenesis approaches. Here, we report that the hypervariable region (HVR), consisting of amino acids 166 to 189 in H-Ras, determines the invasive/migratory signaling program as shown by the exchange of invasive phenotype by swapping HVR sequences between H-Ras and N-Ras. We also demonstrate that the H-Ras-specific additional palmitoylation site at Cys184 is not responsible for the signaling events that distinguish between H-Ras and N-Ras. Importantly, this work identifies the C-terminal HVR, especially the flexible linker domain with two consecutive proline residues Pro173 and Pro174, as a critical domain that contributes to activation of H-Ras and its invasive potential in human breast epithelial cells. The present study sheds light on the structural basis for the Ras isoform-specific invasive program of breast epithelial cells, providing information for the development of agents that specifically target invasion-related H-Ras pathways in human cancer. © 2011 Neoplasia Press, Inc. All rights reserved. | - |
dc.language | English | - |
dc.title | Identification of H-Ras-Specific motif for the activation of invasive signaling program in human breast epithelial cells | - |
dc.type | Article | - |
dc.relation.issue | 2 | - |
dc.relation.volume | 13 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 98 | - |
dc.relation.lastpage | 107 | - |
dc.relation.journaltitle | Neoplasia | - |
dc.identifier.doi | 10.1593/neo.101088 | - |
dc.identifier.wosid | WOS:000286944300002 | - |
dc.identifier.scopusid | 2-s2.0-79951495657 | - |
dc.author.google | Yong H.-Y. | - |
dc.author.google | Hwang J.-S. | - |
dc.author.google | Son H. | - |
dc.author.google | Park H.-I. | - |
dc.author.google | Oh E.-S. | - |
dc.author.google | Kim H.-H. | - |
dc.author.google | Kim D.K. | - |
dc.author.google | Choi W.S. | - |
dc.author.google | Lee B.-J. | - |
dc.author.google | Kim H.-R.C. | - |
dc.author.google | Moon A. | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.date.modifydate | 20190901081003 | - |