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Pharmacophore-based strategy for the development of general and specific scFv biosensors for abused antibiotics

Title
Pharmacophore-based strategy for the development of general and specific scFv biosensors for abused antibiotics
Authors
Cha M.Y.Lee H.Y.Ko Y.Shim H.Park S.B.
Ewha Authors
심현보
SCOPUS Author ID
심현보scopus
Issue Date
2011
Journal Title
Bioconjugate Chemistry
ISSN
1043-1802JCR Link
Citation
vol. 22, no. 1, pp. 88 - 94
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on the pharmacophore of fluoroquinolone antibiotics and the subsequent selection of scFv receptor modules against these antibiotics-based antigens using phage display. The resulting A2 and F9 scFvs bound to their representative antigen with a moderate affinty (KD in micromolar range as determined by surface plasmon resonance). A2 is a specific binder for enrofloxacin and did not cross-react with other fluoroquinolone antibiotics including structurally similar ciprofloxacin, while F9 is a general fluoroquinolone binder that likely bound to the antigen at the common pyridone-carboxylic acid pharmacophore. These scFv-based receptors were successfully applied to the development of one-step fluorescent biosensor which can detect fluoroquinolone antibiotics at concentrations below the level suggested in animal drug application guidelines. The strategy described in this report can be applied to developing convenient field biosensors that can qualitatively detect overused/misused antibiotics in the livestock drinking water. © 2010 American Chemical Society.
DOI
10.1021/bc1004153
Appears in Collections:
일반대학원 > 바이오융합과학과 > Journal papers
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