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Globotriaosylceramide leads to KCa3.1 channel dysfunction: A new insight into endothelial dysfunction in Fabry disease
- Globotriaosylceramide leads to KCa3.1 channel dysfunction: A new insight into endothelial dysfunction in Fabry disease
- Park S.; Kim J.A.; Joo K.Y.; Choi S.; Choi E.-N.; Shin J.-A.; Han K.-H.; Jung S.-C.; Suh S.H.
- Ewha Authors
- 서석효; 정성철; 한기환; 최신규; 박성희
- SCOPUS Author ID
- 서석효; 정성철; 한기환; 최신규; 박성희
- Issue Date
- Journal Title
- Cardiovascular Research
- vol. 89, no. 2, pp. 290 - 299
- SCI; SCIE; SCOPUS
- AimsExcessive endothelial globotriaosylceramide (Gb3) accumulation is associated with endothelial dysfunction and impaired endothelium-dependent relaxation in Fabry disease. In endothelial cells, KCa3.1 channels contribute to endothelium-dependent relaxation. However, the effect of Gb3 on KCa3.1 channels and the underlying mechanisms of Gb3-induced dysfunction are unknown. Herein, we hypothesized that Gb3 accumulation induces KCa3.1 channel dysfunction and aimed to clarify the underlying mechanisms. Methods and resultsThe animal model of Fabry disease, α-galactosidase A (Gla) knockout mice, displayed age-dependent KCa3.1 channel dysfunction. KCa3.1 current and the channel expression were significantly reduced in mouse aortic endothelial cells (MAECs) of aged Gla knockout mice, whereas they were not changed in MAECs of wild-type and young Gla knockout mice. In addition, KCa3.1 current and the channel expression were concentration-dependently reduced in Gb3-treated MAECs. In both Gb3-treated and aged Gla knockout MAECs, extracellular signal-regulated kinase (ERK) and activator protein-1 (AP-1) were down-regulated and repressor element-1 silencing transcription factor (REST) was up-regulated. Gb3 inhibited class III phosphoinositide 3-kinase and decreased intracellular levels of phosphatidylinositol 3-phosphate [PI(3)P]. In addition, endothelium-dependent relaxation was significantly attenuated in Gb3-treated mouse aortic rings. ConclusionGb3 accumulation reduces KCa3.1 channel expression by down-regulating ERK and AP-1 and up-regulating REST and the channel activity by decreasing intracellular levels of PI(3)P. Gb3 thereby evokes KCa3.1 channel dysfunction, and the channel dysfunction in vascular endothelial cells may contribute to vasculopathy in Fabry disease. © 2010 The Author.
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