View : 13 Download: 0

Simple determination of azasetron in rat plasma by column-switching high-performance liquid chromatography

Title
Simple determination of azasetron in rat plasma by column-switching high-performance liquid chromatography
Authors
Um S.Y.Chae S.W.Park H.J.Chung M.W.Choi S.O.Lee H.J.
Ewha Authors
이화정
SCOPUS Author ID
이화정scopus
Issue Date
2010
Journal Title
Journal of Separation Science
ISSN
1615-9306JCR Link
Citation
vol. 33, no. 23-24, pp. 3638 - 3643
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
For the quantification of azasetron in rat plasma samples, a column-switching HPLC method was developed and validated. Following dilution of plasma samples with mobile phase A (17 mM potassium phosphate buffer (pH 3.0)) and simple protein precipitation by addition of perchloric acid (60%), the mixture was directly injected onto the pre-column. After endogenous plasma substances were eluted to waste, the analyte was transferred to the trap column by switching the system. Then, the analyte was back-flushed to the analytical column for separation with mobile phase B (a 22:78 v/v mixture of acetonitrile and 17 mM potassium phosphate buffer (pH 3.0)) and detected at 250 nm using a photodiode array detector. A linear standard curve was obtained in the concentration range of 10-800 ng/mL with the correlation coefficient (r) of 0.9998. The intra- and inter-day precision and accuracy values for azasetron were in the ranges of 0.3-12.9% and 89.7-101.4%, respectively. The method was valid in terms of specificity, precision, and accuracy. In addition, this efficient analytical method was successfully applied to determine plasma concentrations of azasetron following oral administration of azasetron at a dose of 4.0 mg/kg to rats. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
DOI
10.1002/jssc.201000401
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE