Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 편욱범 | * |
dc.date.accessioned | 2016-08-28T12:08:58Z | - |
dc.date.available | 2016-08-28T12:08:58Z | - |
dc.date.issued | 2010 | * |
dc.identifier.issn | 0969-7128 | * |
dc.identifier.other | OAK-7119 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221225 | - |
dc.description.abstract | Hepatocyte growth factor (HGF) has been shown to induce angiogenesis in vivo and has potential as a candidate gene for therapeutic angiogenesis. In vivo, two isoforms of HGF, HGF 723 and HGF 728, consisting of 723 and 728 amino acids, are generated through alternative splicing between exons 4 and 5, but the biological effects of their coexpression have not yet been elucidated. In this study, we generated a series of genomic-complementary DNA (cDNA) hybrids of the HGF gene by inserting various truncated intron 4 into the junction of exons 4 and 5 of HGF cDNA and analyzed the biological activities of these hybrid constructs. We showed that: (1) the hybrid called HGF-X7, which contained 1502 base pairs of intron 4, could drive a higher level of HGF expression than other hybrid constructs and cDNAs of each isoform alone; (2) the pCK vector was most efficient for the gene expression of HGF-X7; (3) coexpression of both isoforms of HGF could more efficiently induce the migration of human umbilical vein endothelial cell (HUVEC) and of the mouse myoblast cell line C 2C 12 myoblasts than a single isoform of HGF and human vascular endothelial growth factor (VEGF) 165 at a given concentration; (4) intramuscular administration of pCK-HGF-X7 resulted in transient and localized HGF expression in the injected muscle without an increase in the HGF protein levels in other tissues including serum; and (5) intramuscular injection of pCK-HGF-X7 could more efficiently increase the number of angiographically recognizable collateral vessels, as well as improve an intra-arterial Doppler wire-measured blood flow in the rabbit model of hindlimb ischemia when compared with the identical vector encoding VEGF 165 gene. These results showed that transfer of the genomic-cDNA hybrid of the HGF gene could be used as a potential therapeutic approach to human vascular diseases. © 2010 Macmillan Publishers Limited All rights reserved. | * |
dc.language | English | * |
dc.title | Naked DNA expressing two isoforms of hepatocyte growth factor induces collateral artery augmentation in a rabbit model of limb ischemia | * |
dc.type | Article | * |
dc.relation.issue | 12 | * |
dc.relation.volume | 17 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1442 | * |
dc.relation.lastpage | 1452 | * |
dc.relation.journaltitle | Gene Therapy | * |
dc.identifier.doi | 10.1038/gt.2010.101 | * |
dc.identifier.wosid | WOS:000285140000004 | * |
dc.identifier.scopusid | 2-s2.0-78650029098 | * |
dc.author.google | Pyun W.-B. | * |
dc.author.google | Hahn W. | * |
dc.author.google | Kim D.-S. | * |
dc.author.google | Yoo W.-S. | * |
dc.author.google | Lee S.-D. | * |
dc.author.google | Won J.-H. | * |
dc.author.google | Rho B.-S. | * |
dc.author.google | Park Z.-Y. | * |
dc.author.google | Kim J.-M. | * |
dc.author.google | Kim S. | * |
dc.contributor.scopusid | 편욱범(6508352922) | * |
dc.date.modifydate | 20240123092816 | * |