Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김희선 | * |
dc.contributor.author | 박은미 | * |
dc.date.accessioned | 2016-08-28T12:08:56Z | - |
dc.date.available | 2016-08-28T12:08:56Z | - |
dc.date.issued | 2010 | * |
dc.identifier.issn | 0022-3042 | * |
dc.identifier.other | OAK-7090 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221200 | - |
dc.description.abstract | Microglia activation plays a pivotal role in neurodegenerative diseases, and thus controlling microglial activation has been suggested as a promising therapeutic strategy for neurodegenerative diseases. In the present study, we showed that ginsenoside Rh1 inhibited inducible nitric oxide synthase, cyclooxygenase-2, and pro-inflammatory cytokine expression in lipopolysaccharide (LPS)-stimulated microglia, while Rh1 increased anti-inflammatory IL-10 and hemeoxygenase-1 (HO-1) expression. Suppression of microglial activation by Rh1 was also observed in the mouse brain following treatment with LPS. Subsequent mechanistic studies revealed that Rh1 inhibited LPS-induced MAPK phosphorylation and nuclear factor-κB (NF-κB)-mediated transcription without affecting NF-κB DNA binding. As the increase of pCREB (cAMP responsive element-binding protein) is known to result in suppression of NF-κB-mediated transcription, we examined whether Rh1 increased pCREB levels. As expected, Rh1 increased pCREB, which was shown to be related to the anti-inflammatory effect of Rh1 because pre-treatment with protein kinase A inhibitors attenuated the Rh1-mediated inhibition of nitric oxide production and the up-regulation of IL-10 and HO-1. Furthermore, treatment of HO-1 shRNA attenuated Rh1-mediated inhibition of nitric oxide and reactive oxygen species production. Through this study, we have demonstrated that protein kinase A and its downstream effector, HO-1, play a critical role in the anti-inflammatory mechanism of Rh1 by modulating pro- and anti-inflammatory molecules in activated microglia. © 2010 The Authors. Journal of Neurochemistry © 2010 International Society for Neurochemistry. | * |
dc.language | English | * |
dc.title | Anti-inflammatory mechanism of ginsenoside Rh1 in lipopolysaccharide- stimulated microglia: Critical role of the protein kinase A pathway and hemeoxygenase-1 expression | * |
dc.type | Article | * |
dc.relation.issue | 6 | * |
dc.relation.volume | 115 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1668 | * |
dc.relation.lastpage | 1680 | * |
dc.relation.journaltitle | Journal of Neurochemistry | * |
dc.identifier.doi | 10.1111/j.1471-4159.2010.07075.x | * |
dc.identifier.wosid | WOS:000284852600032 | * |
dc.identifier.scopusid | 2-s2.0-78649993974 | * |
dc.author.google | Jung J.-S. | * |
dc.author.google | Shin J.A. | * |
dc.author.google | Park E.-M. | * |
dc.author.google | Lee J.-E. | * |
dc.author.google | Kang Y.-S. | * |
dc.author.google | Min S.-W. | * |
dc.author.google | Kim D.-H. | * |
dc.author.google | Hyun J.-W. | * |
dc.author.google | Shin C.-Y. | * |
dc.author.google | Kim H.-S. | * |
dc.contributor.scopusid | 김희선(57191372551) | * |
dc.contributor.scopusid | 박은미(35933416400) | * |
dc.date.modifydate | 20240123095000 | * |