Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 최정윤 | - |
dc.date.accessioned | 2016-08-28T12:08:55Z | - |
dc.date.available | 2016-08-28T12:08:55Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0022-2836 | - |
dc.identifier.other | OAK-7079 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221189 | - |
dc.description.abstract | Abasic (apurinic/apyrimidinic, AP) sites are the most common DNA lesions formed in cells, induce severe blocks to DNA replication, and are highly mutagenic. Human Y-family translesion DNA polymerases (pols) such as pols η, ι, κ, and REV1 have been suggested to play roles in replicative bypass across many DNA lesions where B-family replicative pols stall, but their individual catalytic functions in AP site bypass are not well understood. In this study, oligonucleotides containing a synthetic abasic lesion (tetrahydrofuran analogue) were compared for catalytic efficiency and base selectivity with human Y-family pols η, ι, κ, and REV1 and B-family pols α and δ. Pol η and pol δ/proliferating cell nuclear antigen (PCNA) copied past AP sites quite effectively and generated products ranging from one-base to full-length extension. Pol ι and REV1 readily incorporated one base opposite AP sites but then stopped. Pols κ and α were severely blocked at AP sites. Pol η preferentially inserted T and A; pol ι inserted T, G, and A; pol κ inserted C and A; REV1 preferentially inserted C opposite AP sites. The B-family pols α and δ/PCNA preferentially inserted A (85% and 58%, respectively) consonant with the A-rule hypothesis. Pols η and δ/PCNA were much more efficient in next-base extension, preferably from A positioned opposite an AP site, than pol κ. These results suggest that AP sites might be bypassed with moderate efficiency by single B- and Y-family pols or combinations, possibly by REV1 and pols ι, η, and δ/PCNA at the insertion step opposite the lesion and by pols η, and δ/PCNA at the subsequent extension step. The patterns of the base preferences of human B-family and Y-family pols in both insertion and extension are pertinent to some of the mutagenesis events induced by AP lesions in human cells. © 2010 Elsevier Ltd. | - |
dc.language | English | - |
dc.title | Translesion Synthesis across Abasic Lesions by Human B-Family and Y-Family DNA Polymerases α, δ, η, ι, κ, and REV1 | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 404 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 34 | - |
dc.relation.lastpage | 44 | - |
dc.relation.journaltitle | Journal of Molecular Biology | - |
dc.identifier.doi | 10.1016/j.jmb.2010.09.015 | - |
dc.identifier.wosid | WOS:000284674000003 | - |
dc.identifier.scopusid | 2-s2.0-78049421745 | - |
dc.author.google | Choi J.-Y. | - |
dc.author.google | Lim S. | - |
dc.author.google | Kim E.-J. | - |
dc.author.google | Jo A. | - |
dc.author.google | Guengerich F.P. | - |
dc.contributor.scopusid | 최정윤(57223660142;34973862000) | - |
dc.date.modifydate | 20230627091252 | - |