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Expression Analysis of Proline Metabolism-related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

Title
Expression Analysis of Proline Metabolism-related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions
Authors
Jung Y.Park J.Choi Y.Yang J.-G.Kim D.Kim B.-G.Roh K.Lee D.-H.Auh C.-K.Lee S.
Ewha Authors
이동희
SCOPUS Author ID
이동희scopus
Issue Date
2010
Journal Title
Journal of Integrative Plant Biology
ISSN
1672-9072JCR Link
Citation
vol. 52, no. 10, pp. 891 - 903
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A. stelleri plants treated with 0.2 M mannitol versus mock-treated plants. The most significant changes in the gene expression patterns were in genes defining cellular components or in genes associated with the endomembrane system, stimulus response, stress response, chemical stimulus response, and defense response. The expression patterns of three de novo proline biosynthesis enzymes were evaluated in A. stelleri var. japonica seedlings treated with 0.2 M mannitol, 0.2 M sorbitol, and 0.2 M NaCl. The expression of Δ1-pyrroline-5-carboxylate synthetase was not affected by NaCl stress but was similarly induced by mannitol and sorbitol. The proline dehydrogenase gene, which is known to be repressed by dehydration stress and induced by free L-proline, was induced at an early stage by mannitol treatment, but the level of proline dehydrogenase was increased later by treatment with both mannitol and NaCl. The level of free L-proline accumulation increased progressively in response to treatments with mannitol, sorbitol, and NaCl. Mannitol induced L-proline accumulation more rapidly than NaCl or sorbitol. These findings demonstrate that the osmotic tolerance of the novel halophyte, Arabis stelleri, is associated with the accumulation of L-proline. © 2010 Institute of Botany, Chinese Academy of Sciences.
DOI
10.1111/j.1744-7909.2010.00990.x
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일반대학원 > 바이오융합과학과 > Journal papers
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