View : 13 Download: 0

Novel three-dimensional scaffolds of poly(L -lactic acid) microfibers using electrospinning and mechanical expansion: Fabrication and bone regeneration

Title
Novel three-dimensional scaffolds of poly(L -lactic acid) microfibers using electrospinning and mechanical expansion: Fabrication and bone regeneration
Authors
Shim I.K.Jung M.R.Kim K.H.Seol Y.J.Park Y.J.Park W.H.Lee S.J.
Ewha Authors
이승진심인경
SCOPUS Author ID
이승진scopus
Issue Date
2010
Journal Title
Journal of Biomedical Materials Research - Part B Applied Biomaterials
ISSN
1552-4973JCR Link
Citation
vol. 95, no. 1, pp. 150 - 160
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Poly(L-lactic acid) (PLLA) microfibrous scaffolds with three-dimensional (3D) structures were fabricated using an electrospinning technique with a subsequent mechanical expansion process. To achieve a 3D fibrous structure, the fusion at the contact points of the as-spun PLLA microfibers was avoided using an appropriate binary solvent system of methylene chloride and acetone. The solvent composition was optimized based on the solvent power, volatility, and viscosity (methylene chloride:acetone = 9:1 volume ratio). The final 3D structure of the electrospun scaffolds was obtained after mechanical expansion of the electrospun microfibrous mats. The pore sizes of the scaffolds were controlled by varying the degree of expansion of the nonbonded microfibrous mats, and they were in the range of several microns up to 400 μm. The 3D scaffolds were examined for their morphological properties and their potential use for the proliferation of osteoblasts. Generally recognized electrospun 2D nanofibrous membranes were also tested in order to compare the cell behaviors using different scaffold geometries. The 3D scaffolds demonstrated a high level of osteoblast proliferation (1.8-fold higher than nanofibrous membranes in a week). The osteoblasts actively penetrated the inside of the 3D scaffold and showed a spatial cell distribution, as confirmed by SEM and H&E staining, while a monolayer formed in the case of the 2D nanofibrous membranes with limited cell infiltration. In vivo results further showed that 3D electrospun microfibrous matrices were a favorable substrate for cell infiltration and bone formation after 2 and 4 weeks, using a rabbit calvarial defect model. In this study, the 3D microfibrous PLLA scaffolds fabricated using electrospinning techniques might be an innovative addition to tissue engineering applications. © 2010 Wiley Periodicals, Inc.
DOI
10.1002/jbm.b.31695
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE