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Production of polyclonal antibodies against the tegument of sparganum (Plerocercoid of Spirometra mansoni) and its immunolocalization

Title
Production of polyclonal antibodies against the tegument of sparganum (Plerocercoid of Spirometra mansoni) and its immunolocalization
Authors
Yang H.-J.
Ewha Authors
양현종
SCOPUS Author ID
양현종scopus
Issue Date
2010
Journal Title
Korean Journal of Parasitology
ISSN
0023-4001JCR Link
Citation
vol. 48, no. 2, pp. 183 - 185
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
In a previous study, the author developed a method for separation of the tegument of spargana (plerocercoids of Spirometra mansoni) from the parenchyme using urea. The present study, as a next step, was performed to evaluate which molecules are present in the outer tegument. Two major proteins, 180 and 200 kDa, are present in the tegument and we could make polyclonal antibodies against these molecules. Their immunolocalization was processed and the outermost layer of the spargana showed strong positive staining. Conclusively, we could confirm that the 180 and 200 kDa molecules might be tightly bound membrane proteins in the tegument of spargana.
DOI
10.3347/kjp.2010.48.2.183
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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