View : 592 Download: 0

Ginsenoside Rh1 suppresses inducible nitric oxide synthase gene expression in IFN-γ-stimulated microglia via modulation of JAK/STAT and ERK signaling pathways

Title
Ginsenoside Rh1 suppresses inducible nitric oxide synthase gene expression in IFN-γ-stimulated microglia via modulation of JAK/STAT and ERK signaling pathways
Authors
Jung J.-S.Kim D.-H.Kim H.-S.
Ewha Authors
김희선
SCOPUS Author ID
김희선scopus
Issue Date
2010
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
Biochemical and Biophysical Research Communications vol. 397, no. 2, pp. 323 - 328
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Microglial activation plays an important role in the pathogenesis of various neurodegenerative diseases by producing neurotoxic factors, such as pro-inflammatory cytokines and nitric oxide (NO). In the present study, we found that protopanaxatriol ginsenoside Rh1 suppresses NO, ROS, and TNF-α production in IFN-γ-stimulated BV2 microglial cells. Rh1 inhibited the mRNA and protein expression of iNOS and TNF-α. To determine the regulatory mechanism of iNOS gene expression by Rh1, promoter analysis was performed. Rh1 significantly suppressed IFN-γ-induced iNOS promoter activity by inhibiting DNA binding of several transcription factors, such as NF-jB, IRF-1, and STAT1. Furthermore, Rh1 inhibited the phosphorylation of JAK1, STAT1, STAT3, and ERK, which are upstream signaling molecules for IFNγ-induced iNOS gene expression. The present study demonstrates that Rh1 inhibits IFN-γ-induced JAK/ STAT and ERK signaling pathways and downstream transcription factors, and thereby iNOS gene expression. Therefore, the inhibition of microglial activation by ginsenoside Rh1 may provide potential therapeutic strategy for various neuroinflammatory diseases. © 2010 Elsevier Inc.
DOI
10.1016/j.bbrc.2010.05.117
Appears in Collections:
의과대학 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

BROWSE