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Melphalan inhibits adenoma development through modulating the expression of K-ras-specific markers in K-ras Tg mice

Title
Melphalan inhibits adenoma development through modulating the expression of K-ras-specific markers in K-ras Tg mice
Authors
Lee S.Choi H.Kim E.Kim H.Park Y.-H.Yu D.-Y.Yoon S.-J.Kim J.Sheen Y.Park S.-N.Yoon D.-Y.
Ewha Authors
신윤용
SCOPUS Author ID
신윤용scopus
Issue Date
2010
Journal Title
International Journal of Oncology
ISSN
1019-6439JCR Link
Citation
vol. 37, no. 1, pp. 219 - 228
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
In previous research, we focused on the discovery of K-ras biomarkers, and effects of genotoxic carcinogens on their expression were investigated in this study. It is well-known that mutated K-ras gene is involved in ∼30% of human cancers such as lung cancer. To search for K-ras oncogene-induced modulators in lung tissues of K-ras transgenic mice, we analyzed K-ras-specific genes and proteins related to cancer development, signal transduction, inflammation as well as tumor suppression in a previous study. In this study, we investigated the modulating effects of genotoxic carcinogen treatment on expression of K-ras-dependent modulated genes and proteins in lung tissues of K-ras Tg mice. In order to evaluate candidate K-ras markers modulated by genotoxic stress and to investigate whether a genotoxic carcinogen would enhance or inhibit carcinogenesis in lung tissues of the K-ras Tg mice, the anti-cancer drug melphalan was intraperitoneally injected into K-ras Tg mice every two days for four weeks. RT-qPCR and proteomics analyses were performed in order to confirm whether K-ras-specific biomarkers would be modulated by melphalan treatment in K-ras Tg mice. The decreased adenomas were histopathologically observed and K-ras expression was suppressed in melphalan-treated K-ras Tg mice. Melphalan also recovered the expression of K-ras-dependent modulated biomarkers. These results suggest that melphalan inhibits carcinogenesis via modulating K-ras-specific genes and proteins expressed in the lung tissues of K-ras Tg mice.
DOI
10.3892/ijo-00000670
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약학대학 > 약학과 > Journal papers
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