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Immunophenotypic features of granulocytes, monocytes, and blasts in myelodysplastic syndromes

Title
Immunophenotypic features of granulocytes, monocytes, and blasts in myelodysplastic syndromes
Authors
Moon H.W.Huh J.W.Lee M.Hong K.S.Chung W.S.
Ewha Authors
홍기숙이미애정화순허정원
SCOPUS Author ID
홍기숙scopus; 이미애scopus; 정화순scopus; 허정원scopus
Issue Date
2010
Journal Title
Korean Journal of Laboratory Medicine
ISSN
1598-6535JCR Link
Citation
vol. 30, no. 2, pp. 97 - 104
Indexed
SCOPUS WOS scopus
Abstract
Background: Despite the diagnostic utility of immunophenotyping for myelodysplastic syndromes (MDS), it has not been widely performed, and reports on this are absent in Korea. We aimed to evaluate the immunophenotypic features of non-blastic granulocytes, monocytes, and blasts in patients with MDS and non-clonal disorders using routine flow cytometry (FCM). Moreover, we evaluated the phenotypic abnormalities of mature cells in leukemic patients. Methods: Marrow aspirates from 60 patients, including 18 with MDS, 18 with leukemia, and 24 with non-clonal disorders (control group), were analyzed using FCM. Blasts, non-blast myeloid cells, and monocytes were gated based on CD45 expression and side scatter (SSC). The phenotypes were then compared among the 3 groups. Results: Compared to non-clonal disorders, the granulocytic lineages of MDS showed decreased SSC (P=0.005), increased CD45 intensity (P=0.020), decreased CD10-positive granulocytes (P= 0.030), and a higher CD56-positive rate (P=0.005). It is noteworthy that similar results were obtained in the leukemia group, and these findings were not related to the phenotypes of the leukemic cells. Using blast and monocytic gating, useful parameters for generating a differential diagnosis were not found. Conclusions: Gating the granulocytic region is a relatively easy method for MDS immunophenotyping. Among the parameters studied, SSC, CD10, and CD56 were the most useful for differentiating MDS from non-clonal disorders. While immunophenotypic changes in MDS appear to be useful for differentiating MDS from non-clonal disorders, these changes were also noted in the mature cells of leukemic patients.
DOI
10.3343/kjlm.2010.30.2.97
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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