Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서석효 | * |
dc.contributor.author | 최신규 | * |
dc.contributor.author | 박성희 | * |
dc.date.accessioned | 2016-08-28T12:08:09Z | - |
dc.date.available | 2016-08-28T12:08:09Z | - |
dc.date.issued | 2010 | * |
dc.identifier.issn | 0024-3205 | * |
dc.identifier.other | OAK-6505 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220729 | - |
dc.description.abstract | Aims: This study examined the effects of oxidized low-density lipoprotein (LDL) and its major lipid constituent lysophosphatidylcholine (LPC) on nonselective cation (NSC) current and its inhibitory contribution to LPC-induced cytotoxicity in cultured human umbilical endothelial cells (HUVECs). Main methods: Patch-clamp technique and the resazurin-based cell viability assay were used. Key findings: In voltage-clamped cells, oxidized LDL or LPC slowly activated NSC current. NSC current was also activated by loading cells with Ca2+ solution buffered at various concentrations using a patch pipette or by applying the sarcoplasmic reticulum Ca2+ pump blocker 2,5-di-t-butyl-1,4-benzohydroquinone (BHQ), the metabolic inhibitor CN- or the hydroperoxide donor tert-butyl hydroperoxide (TBHP). On the contrary, when intracellular Ca2+ was strongly buffered with 12mM BAPTA or cells were loaded with superoxide dismutase using a patch pipette, LPC or BHQ did not activate NSC current. Furthermore, NSC current activated by LPC, TBHP or CN- was inhibited by the antioxidant tempol or extracellular Ca2+ depletion and NSC current activated by intracellular Ca2+ was further augmented by oxidized LDL or LPC. LPC or oxidized LDL released Ca2+ from intracellular stores and further enhanced store-operated Ca2+ entry. LPC-induced cytotoxicity was augmented by inhibiting Ca2+ influx and NO synthesis. Significance: Oxidized LDL or its main component LPC activated Ca2+-permeable NSC current via releasing Ca2+ from intracellular stores and producing ROS and thereby increased Ca2+ influx. Ca2+ influx through NSC channel might protect endothelial cells by producing NO. © 2010 Elsevier Inc. | * |
dc.language | English | * |
dc.title | Modulation of nonselective cation current by oxidized LDL and lysophosphatidylcholine and its inhibitory contribution to endothelial damage | * |
dc.type | Article | * |
dc.relation.issue | 19-20 | * |
dc.relation.volume | 86 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 733 | * |
dc.relation.lastpage | 739 | * |
dc.relation.journaltitle | Life Sciences | * |
dc.identifier.doi | 10.1016/j.lfs.2010.03.005 | * |
dc.identifier.wosid | WOS:000277192500006 | * |
dc.identifier.scopusid | 2-s2.0-77951934149 | * |
dc.author.google | Liang G.H. | * |
dc.author.google | Park S. | * |
dc.author.google | Kim M.Y. | * |
dc.author.google | Kim J.A. | * |
dc.author.google | Choi S. | * |
dc.author.google | Suh S.H. | * |
dc.contributor.scopusid | 서석효(55666113100) | * |
dc.contributor.scopusid | 최신규(12783276200) | * |
dc.contributor.scopusid | 박성희(8848996000) | * |
dc.date.modifydate | 20240220100710 | * |