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Comparison of microbial communities in petroleum-contaminated groundwater using genetic and metabolic profiles at Kyonggi-Do, South Korea

Title
Comparison of microbial communities in petroleum-contaminated groundwater using genetic and metabolic profiles at Kyonggi-Do, South Korea
Authors
Lee E.-H.Kim J.Kim J.-Y.Koo S.-Y.Lee S.-D.Ko K.-S.Ko D.-C.Yum B.-W.Cho K.-S.
Ewha Authors
조경숙이상돈이은희
SCOPUS Author ID
조경숙scopus; 이상돈scopus; 이은희scopusscopus
Issue Date
2010
Journal Title
Environmental Earth Sciences
ISSN
1866-6280JCR Link
Citation
vol. 60, no. 2, pp. 371 - 382
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
This research has been performed to determine the differences in microbial communities according to physicochemical properties such as concentrations of volatile aromatic hydrocarbons such as benzene, toluene, ethylbenzene, and xylene (BTEX), dissolved oxygen (DO), electron acceptors, etc., in oil-contaminated groundwaters at Kyonggi-Do, South Korea. The properties of bacterial and microbial communities were analyzed by 16S polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) fingerprinting method and community-level physiological profiling (CLPP) using Eco-plate, respectively. Based on the DGGE fingerprints, the similarities of bacterial community structures were high with similar DO levels, and low with different DO levels. Whereas the dominant bacterial groups in GW13 (highest BTEX and lowest DO) were acidobacteria, α-proteobacteria, β-proteobacteria, γ-proteobacteria, δ-proteobacteria, and spirochetes, those in GW7 (highest BTEX and highest DO) were actinobacteria, α-proteobacteria, β-proteobacteria, γ-proteobacteria, δ-proteobacteria, and sphingobacteria. Based on the CLPP results, the groundwater samples were roughly divided into three groups: above 4 mg/L in DO (group 1: GW3 and GW7), below 4 mg/L in DO (group 2: GW8, W1, W2, W3, and BH10), and highly contaminated with BTEX (group 3: GW13). Shannon index showed that the microbial diversities and equitabilities were higher in shallower aquifer samples. Overall, this study verified that the greatest influencing factors on microbial/bacterial communities in groundwaters were DO and carbon sources, although BTEX concentration was one of the major factors. © 2009 Springer-Verlag.
DOI
10.1007/s12665-009-0181-7
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엘텍공과대학 > 환경공학전공 > Journal papers
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