View : 14 Download: 0

Mesenchymal stromal cells inhibit graft-versus-host disease of mice in a dose-dependent manner

Title
Mesenchymal stromal cells inhibit graft-versus-host disease of mice in a dose-dependent manner
Authors
Joo S.-Y.Cho K.-A.Jung Y.-J.Kim H.-S.Park S.-Y.Choi Y.-B.Hong K.-M.Woo S.-Y.Seoh J.-Y.Cho S.J.Ryu K.-H.
Ewha Authors
서주영유경하우소연조수진조경아
SCOPUS Author ID
서주영scopus; 유경하scopus; 우소연scopus; 조수진scopus; 조경아scopus
Issue Date
2010
Journal Title
Cytotherapy
ISSN
1465-3249JCR Link
Citation
vol. 12, no. 3, pp. 361 - 370
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Background aims. Graft-versus-host disease (GvHD) remains a major complication after allogeneic hematopoietic cell transplantation (HCT). Recent literature demonstrates a potential benefit of human mesenchymal stromal cells (MSC) for the treatment of refractory GvHD; however, the optimal dose remains uncertain. We set out to develop an animal model that can be used to study the effect of MSC on GvHD. Methods. A GvHD mouse model was established by transplanting C3H/he donor bone marrow (BM) cells and spleen cells into lethally irradiated BALB/c recipient mice. MSC were obtained from C3H/he mice and the C3H/10T1/2 murine MSC line. Results. The mRNA expression of Foxp3 in regional lymph nodes (LN) localized with T cells was markedly increased by the addition of C3H10T1/2 cells in a real-time polymerase chain reaction (PCR). Using a mixed lymphocyte reaction, we determined the optimal splenocyte proliferation inhibition dose (MSC:splenocyte ratios 1:2 and 1:1). Three different C3H10T1/2 cell doses (low, 0.5 × 106, intermediate, 1 × 10 6, and high, 2 × 106) with a consistent splenocyte dose (1 × 106) were evaluated for their therapeutic potential in an in vivo GvHD model. The clinical and histologic GvHD score and KaplanMeier survival rate were improved after MSC transplantation, and these results demonstrated a dose-dependent inhibition. Conclusions. We conclude that MSC inhibit GvHD in a dose-dependent manner in this mouse model and this model can be used to study the effects of MSC on GvHD.
DOI
10.3109/14653240903502712
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE