Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤영대 | - |
dc.date.accessioned | 2016-08-28T12:08:56Z | - |
dc.date.available | 2016-08-28T12:08:56Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | OAK-6360 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220609 | - |
dc.description.abstract | The T cell-specific tyrosine kinase, p56lck, plays crucial roles in T cell receptor (TCR)-mediated T cell activation. Here, we report that SOCS-6 (suppressor of cytokine signaling-6) is a negative regulator of p56 lck. SOCS-6 was identified as a protein binding to the kinase domain of p56lck through yeast two-hybrid screening. SOCS-6 bound specifically to p56lck (F505), which mimics the active form of p56lck, but not to wild type p56lck. In Jurkat Tcells, SOCS-6 binding to p56lck was detected 1-2 h after TCR stimulation. Confocal microscopy showed that upon APC-T cell conjugation, SOCS-6 was recruited to the immunological synapse and colocalized with the active form of p56lck. SOCS-6 promoted p56lck ubiquitination and its subsequent targeting to the proteasome. Moreover, SOCS-6 overexpression led to repression of TCR-dependent interleukin-2 promoter activity. These results establish that SOCS-6 acts as a negative regulator of T cell activation by promoting ubiquitin-dependent proteolysis. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. | - |
dc.language | English | - |
dc.title | SOCS-6 negatively regulates T cell activation through targeting p56 lck to proteasomal degradation | - |
dc.type | Article | - |
dc.relation.issue | 10 | - |
dc.relation.volume | 285 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 7271 | - |
dc.relation.lastpage | 7280 | - |
dc.relation.journaltitle | Journal of Biological Chemistry | - |
dc.identifier.doi | 10.1074/jbc.M109.073726 | - |
dc.identifier.wosid | WOS:000275415600039 | - |
dc.identifier.scopusid | 2-s2.0-77951240458 | - |
dc.author.google | Choi Y.B. | - |
dc.author.google | Son M. | - |
dc.author.google | Park M. | - |
dc.author.google | Shin J. | - |
dc.author.google | Yun G. | - |
dc.contributor.scopusid | 윤영대(7201731033) | - |
dc.date.modifydate | 20200901081003 | - |