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Phage display selection of EGFR-specific antibodies by capture-sandwich panning

Title
Phage display selection of EGFR-specific antibodies by capture-sandwich panning
Authors
Ki M.K.Kang K.J.Shim H.
Ewha Authors
심현보
SCOPUS Author ID
심현보scopus
Issue Date
2010
Journal Title
Biotechnology and Bioprocess Engineering
ISSN
1226-8372JCR Link
Citation
vol. 15, no. 1, pp. 152 - 156
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Sandwich ELISA experiment requires two mutually compatible affinity reagents, typically antibodies, that are highly sensitive and specific to the target analyte in its native conformation, and whose epitopes do not overlap each other's. Finding or developing a pair of antibodies that meet these requirements can be a challenge and many otherwise useful antibodies fail in sandwich ELISA-format analysis. In order to discover sandwich immunoassay-compatible antibodies for epidermal growth factor receptor (EGFR), we first immobilized cetuximab, a high-affinity monoclonal antibody against EGFR, on a plastic surface and affinity-captured EGFR in A431 cell lysate. Panning of a phage antibody library on the surface-captured antigen yielded a couple of antibody clones that can be paired with cetuximab and also with each other in the sandwich-format immunoassay. This provides a potentially useful strategy for the development of sandwich ELISA reagents or antibodies against antigens that are hard to purify. © KSBB.
DOI
10.1007/s12257-009-3080-6
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일반대학원 > 바이오융합과학과 > Journal papers
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