View : 15 Download: 4

The adaptor protein LAD/TSAd mediates laminin-dependent T cell migration via association with the 67 kDa laminin binding protein

Title
The adaptor protein LAD/TSAd mediates laminin-dependent T cell migration via association with the 67 kDa laminin binding protein
Authors
Park E.Choi Y.Ahn E.Park I.Yun G.
Ewha Authors
윤영대박인영
SCOPUS Author ID
윤영대scopus; 박인영scopus
Issue Date
2009
Journal Title
Experimental and Molecular Medicine
ISSN
1226-3613JCR Link
Citation
vol. 41, no. 10, pp. 728 - 736
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
The adaptor protein, LAD/TSAd, plays essential roles in T cell activation. To further understand the functions of this protein, we performed yeast two-hybrid screening using TSAd as bait and identified 67 kDa laminin binding protein (LBP) as the interacting partner. Subsequently, TSAd-LBP interaction was confirmed in D1.1 T cell line. Upon costimulation by T cell receptor (TCR) plus laminin crosslinking or TCR plus integrin α6 crosslinking, LBP was coimmunoprecipitated with TSAd. Moreover, TCR plus laminin costimulation- dependent T cell migration was enhanced in D1.1 T cells overexpressing TSAd but was disrupted in D1.1 cells overexpressing dominant negative form of TSAd or TSAd shRNA. These data show that, upon TCR plus integrin costimulation, TSAd associates with LBP and mediates T lymphocyte migration.
DOI
10.3858/emm.2009.41.10.079
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
Files in This Item:
The adaptor protein LAD TSAd mediates laminin-dependent T cell migration via association with the 67 kDa laminin binding protein.pdf(419.78 kB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE