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Critical role of s465 in protein kinase C-increased rat glutamate transporter type 3 activity

Title
Critical role of s465 in protein kinase C-increased rat glutamate transporter type 3 activity
Authors
Baik H.J.Huang Y.Washington J.M.Zuo Z.
Ewha Authors
백희정
SCOPUS Author ID
백희정scopus
Issue Date
2009
Journal Title
International Journal of Neuroscience
ISSN
0020-7454JCR Link
Citation
vol. 119, no. 9, pp. 1419 - 1428
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Glutamate transporters, also called excitatory amino acid transporters (EAATs), uptake extracellular glutamate and regulate neurotransmission. Activation of protein kinase C (PKC) increases the activity of EAAT type 3 (EAAT3), the major neuronal EAAT. We designed this study to determine which amino acid residue(s) in EAAT3 may be involved in this PKC effect. Selective potential PKC phosphorylation sites were mutated. These EAAT3 mutants were expressed in the Xenopus oocytes. Phorbol 12-myristate 13-acetate, a PKC activator, significantly increased wild-type EAAT3 activity. Mutation of serine 465 to alanine or aspartic acid, but not the mutation of threonine 5 to alanine, abolished PKC-increased EAAT3 activity. Our results suggest a critical role of serine 465 in the increased EAAT3 activity by PKC activation. © 2009 Informa UK Ltd All rights reserved.
DOI
10.1080/00207450903014783
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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