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Development and application of a centrifugation-plating method to study the biodiversity of Bacillus species in rice products
- Title
- Development and application of a centrifugation-plating method to study the biodiversity of Bacillus species in rice products
- Authors
- Oh M.-H.; Cox J.M.
- Ewha Authors
- 오미화
- Issue Date
- 2010
- Journal Title
- Food Control
- ISSN
- 0956-7135
- Citation
- Food Control vol. 21, no. 1, pp. 7 - 12
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- A centrifugation-plating method was developed, using amylase and Tween 80 pre-treatment, for detection and enumeration of Bacillus spp. in rice products. The high sensitivity of this method improved detection of a variety of Bacillus species in rice products compared to the spread-plate method. Bacillus spp. were detected in 33 out of 35 raw rice samples with the centrifugation-plating method, but only 13 samples using the spread-plating method, even though 1 mL of a 10-1 sample dilution was used, to increase sensitivity of the method. Known toxigenic or potentially toxigenic species (Bacillus cereus/Bacillus thuringiensis, Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus) were those most frequently found in both raw and cooked rice. © 2009.
- DOI
- 10.1016/j.foodcont.2009.03.008
- Appears in Collections:
- 공과대학 > 식품생명공학과 > Journal papers
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