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Development and application of a centrifugation-plating method to study the biodiversity of Bacillus species in rice products

Title
Development and application of a centrifugation-plating method to study the biodiversity of Bacillus species in rice products
Authors
Oh M.-H.Cox J.M.
Ewha Authors
오미화
Issue Date
2010
Journal Title
Food Control
ISSN
0956-7135JCR Link
Citation
vol. 21, no. 1, pp. 7 - 12
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A centrifugation-plating method was developed, using amylase and Tween 80 pre-treatment, for detection and enumeration of Bacillus spp. in rice products. The high sensitivity of this method improved detection of a variety of Bacillus species in rice products compared to the spread-plate method. Bacillus spp. were detected in 33 out of 35 raw rice samples with the centrifugation-plating method, but only 13 samples using the spread-plating method, even though 1 mL of a 10-1 sample dilution was used, to increase sensitivity of the method. Known toxigenic or potentially toxigenic species (Bacillus cereus/Bacillus thuringiensis, Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus) were those most frequently found in both raw and cooked rice. © 2009.
DOI
10.1016/j.foodcont.2009.03.008
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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