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Insulin-like growth factor-I induces androgen receptor activation in differentiating C2C12 skeletal muscle cells

Title
Insulin-like growth factor-I induces androgen receptor activation in differentiating C2C12 skeletal muscle cells
Authors
Kim H.J.Lee W.J.
Ewha Authors
이원준
SCOPUS Author ID
이원준scopus
Issue Date
2009
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 28, no. 3, pp. 189 - 194
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
The modulating effect of IGF-I on the regulation of AR gene expression and activation in skeletal muscle cells remains poorly understood. In this study, the effects of IGF-I treatment on AR induction and activation in the absence of AR ligands were examined. Differentiating C2C12 cells were treated with different concentrations (0-250 ng/ml) of IGF-I or for various periods of time (0-60 min) of 250 ng/ml IGF-I. Treatment of C2C12 cells with IGF-I resulted in a dose- and time-dependent increase in total AR and phosphorylated AR (Ser 213). IGF-I treatment also led to significantly increased AR mRNA expression when compared with the control. The levels of skeletal α-actin and myogenin mRNA, known target genes of AR, were also significantly upregulated after 5 or 10 min of treatment with IGF-I. Confocal images revealed that IGF-I stimulated nuclear localization of AR in the absence of ligands. In addition, an electrophoretic mobility shift assay indicated that IGF-I stimulated the AR DNA binding activity in a time-dependent manner. The present results suggest that IGF-I stimulates the expression and activation of AR by ligand-independent mechanism in differentiating C2C12 mouse skeletal muscle cells. © The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2009.
DOI
10.1007/s10059-009-0118-8
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신산업융합대학 > 체육과학부 > Journal papers
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