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Partial carotid ligation is a model of acutely induced disturbed flow, leading to rapid endothelial dysfunction and atherosclerosis

Title
Partial carotid ligation is a model of acutely induced disturbed flow, leading to rapid endothelial dysfunction and atherosclerosis
Authors
Nam D.Ni C.-W.Rezvan A.Suo J.Budzyn K.Llanos A.Harrison D.Giddens D.Jo H.
Ewha Authors
조한중
Issue Date
2009
Journal Title
American Journal of Physiology - Heart and Circulatory Physiology
ISSN
0363-6135JCR Link
Citation
vol. 297, no. 4, pp. H1535 - H1543
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Atherosclerosis is closely associated with disturbed flow characterized by low and oscillatory shear stress, but studies directly linking disturbed flow to atherogenesis is lacking. The major reason for this has been a lack of an animal model in which disturbed flow can be acutely induced and cause atherosclerosis. Here, we characterize partial carotid ligation as a model of disturbed flow with characteristics of low and oscillatory wall shear stress. We also describe a method of isolating intimal RNA in sufficient quantity from mouse carotid arteries. Using this model and method, we found that partial ligation causes upregulation of proatherogenic genes, downregulation of antiatherogenic genes, endothelial dysfunction, and rapid atherosclerosis in 2 wk in a p47phox-dependent manner and advanced lesions by 4 wk. We found that partial ligation results in endothelial dysfunction, rapid atherosclerosis, and advanced lesion development in a physiologically relevant model of disturbed flow. It also allows for easy and rapid intimal RNA isolation. This novel model and method could be used for genome-wide studies to determine molecular mechanisms underlying flow-dependent regulation of vascular biology and diseases. Copyright © 2009 the American Physiological Society.
DOI
10.1152/ajpheart.00510.2009
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일반대학원 > 바이오융합과학과 > Journal papers
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