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Chitosan nano-/microfibrous double-layered membrane with rolled-up three-dimensional structures for chondrocyte cultivation

Title
Chitosan nano-/microfibrous double-layered membrane with rolled-up three-dimensional structures for chondrocyte cultivation
Authors
In K.S.Won H.S.Sang Y.L.Sang H.L.Seong J.H.Myung C.L.Lee S.J.
Ewha Authors
이승진심인경
SCOPUS Author ID
이승진scopus
Issue Date
2009
Journal Title
Journal of Biomedical Materials Research - Part A
ISSN
1549-3296JCR Link
Citation
vol. 90, no. 2, pp. 595 - 602
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
With an aim to mimic natural extracellular matrix, we fabricated the nano- and microfibrous matrix with chitosan by electrospinning nanofibers onto predefined microfibrous mesh for effective chondrocytes cultivation. Rolling the double-layered nano-/microfibrous membranes produced three-dimensional (3-D) scaffolds that exhibited the interconnected open pore structure in their scanning electron microscopy images. In vitro chondrocyte culture experiment showed that this nano-/microfibrous 3-D matrix provided a significantly greater microenvironment for chondrocytes to proliferate and produce glycosaminoglycan as compared with only microfibrous 3-D matrix. This difference could be explained by the result on 2-D membrane, where chitosan nanofibrous surface substantially facilitated the cellular attachment and proliferation, and efficiently prevented phenotypic changes of chondrocytes, when compared with chitosan microfibrous membrane and film. In this regard, the nano-/microfibrous 3-D matrix we fabricated in this study would possess a great potential as a system for effective chondrocyte cultivation and also for application to cartilage regeneration therapy. © 2008 Wiley Periodicals, Inc.
DOI
10.1002/jbm.a.32109
Appears in Collections:
약학대학 > 약학과 > Journal papers
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