View : 141 Download: 0

Full metadata record

DC Field Value Language
dc.contributor.author오상석-
dc.contributor.author오미화-
dc.date.accessioned2016-08-28T12:08:16Z-
dc.date.available2016-08-28T12:08:16Z-
dc.date.issued2009-
dc.identifier.issn0362-028X-
dc.identifier.otherOAK-5690-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/220196-
dc.description.abstractThe objective of this study was to develop a novel technique for parallel analysis of eight important foodborne microbes using capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) coupled with multiplex PCR. Specific primers for multiplex PCR amplification of the 16S rRNA gene were designed, corresponding to eight species of bacteria, including Escherichia coli, Clostridium perfringens, Campylobacter jejuni, Salmonella enterica, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Bacillus cereus, for the species-specific identification and optimal separation of their PCR products in subsequent analysis by CE-SSCP. Multiplex PCR conditions including annealing temperature, extension time, the number of PCR cycles, and primer concentrations were then optimized for simultaneous detection of all target foodborne bacteria. The diagnostic system using CE-SSCP combined with multiplex PCR developed here can be used for rapid investigation of causative agents of foodborne illness. The simplicity and high sensitivity of the method may lead to improved management of safety and illness related to food.-
dc.languageEnglish-
dc.titleSimultaneous identification of seven foodborne pathogens and Escherichia coli (pathogenic and nonpathogenic) using capillary electrophoresis-based single-strand conformation polymorphism coupled with multiplex PCR-
dc.typeArticle-
dc.relation.issue6-
dc.relation.volume72-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage1262-
dc.relation.lastpage1266-
dc.relation.journaltitleJournal of Food Protection-
dc.identifier.wosidWOS:000266956000013-
dc.identifier.scopusid2-s2.0-67650076632-
dc.author.googleOh M.-H.-
dc.author.googlePaek S.-H.-
dc.author.googleShin G.W.-
dc.author.googleKim H.-Y.-
dc.author.googleJung G.Y.-
dc.author.googleOh S.-
dc.contributor.scopusid오상석(7404104074)-
dc.date.modifydate20200911081002-
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE