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dc.contributor.author조경숙-
dc.contributor.author이은희-
dc.date.accessioned2016-08-28T12:08:09Z-
dc.date.available2016-08-28T12:08:09Z-
dc.date.issued2009-
dc.identifier.issn0254-8704-
dc.identifier.otherOAK-5348-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/220128-
dc.description.abstractRhodococcus sp. EH831 is a microbial species that can degrade volatile organic compounds. We optimized a method for monitoring quantitative real-time PCR (qRT-PCR) of EH831 that was incorporated into a polyurethane (PU) biofilter. When the genomic DNA of EH831 was directly extracted from a PU sample with immobilized EH831, the recovery efficiency was very low due to DNA absorption into the PU. DNA amplification during PCR was also inhibited by PU impurities. Therefore, a pre-treatment step was necessary. We successfully recovered cells from the PU by squeezing the matrix, adding sterilized water, and vortexing. The recovery efficiency ranged from 105 to 144%, and there was no statistically significant difference. We designed a novel TaqMan probe for EH831 and demonstrated its high specificity for EH831. The detection range for EH831 was 105-1011 CFU ml-1. The method described in this study can be used to investigate the relationship between quantitative analysis of Rhodococcus sp. EH831 and PU biofilter performance. © Triveni Enterprises, Lucknow (India).-
dc.languageEnglish-
dc.titleApplication of quantitative real-time PCR for quantification of Rhodococcus sp. EH831 in a polyurethane biofilter-
dc.typeArticle-
dc.relation.issue1-
dc.relation.volume30-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage155-
dc.relation.lastpage159-
dc.relation.journaltitleJournal of Environmental Biology-
dc.identifier.wosidWOS:000262706900025-
dc.identifier.scopusid2-s2.0-59349102118-
dc.author.googleLee E.H.-
dc.author.googleCho K.S.-
dc.author.googleRyu H.W.-
dc.contributor.scopusid조경숙(7403957095)-
dc.contributor.scopusid이은희(55773710100;56141882500)-
dc.date.modifydate20200227081002-
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엘텍공과대학 > 환경공학전공 > Journal papers
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