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Involvement of pro-phenoloxidase 3 in lamellocyte-meidated spontaneous melanization in Drosophila

Title
Involvement of pro-phenoloxidase 3 in lamellocyte-meidated spontaneous melanization in Drosophila
Authors
Nam H.-J.Jang I.-H.Asano T.Lee W.-J.
Ewha Authors
이원재
Issue Date
2008
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 26, no. 6, pp. 606 - 610
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Phenoloxidase (PO), a melanin-forming enzyme around the foreign bodies, is an important component of the host defense system in invertebrates. Pro-PO is the enzymatically inactive zymogen form of PO. In the Drosophila genome, three Pro-PO isoforms have been identified to date. These include Pro-PO1 and 2, which are primarily expressed in crystal cells, and Pro-PO3, which is predominantly found in the lamellocytes. In this study, we demonstrated that Drosophila Pro-PO3, but not Pro-PO1 or 2, is enzymatically active in its zymogen form. These findings were evidenced by spectacular melanin forming capacities of various cells and tissues that overexpressed these proenzymes. Furthermore, the melanization phenotype observed in the lamellocyte-enriched hop Tum-1 mutant was drastically reduced in the absence of PPO3, indicating that PPO3 plays a major role in the lamellocyte-mediated spontaneous melanization process. Taken together, these findings indicate that the biochemical properties, activation mode and in vivo role of Pro-PO3 are likely distinct from those of the other two Pro-PO enzymes involved in Drosophila physiology. © 2008 KSMCB.
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일반대학원 > 바이오융합과학과 > Journal papers
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