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Glucose/oxygen deprivation and reperfusion upregulate SNAREs and complexin in organotypic hippocampal slice cultures

Title
Glucose/oxygen deprivation and reperfusion upregulate SNAREs and complexin in organotypic hippocampal slice cultures
Authors
Park S.J.Jung Y.J.Kim Y.A.Lee-Kang J.H.Lee K.E.
Ewha Authors
이지희이경은정연주
SCOPUS Author ID
이지희scopus; 이경은scopus; 정연주scopus
Issue Date
2008
Journal Title
Neuropathology
ISSN
0919-6544JCR Link
Citation
vol. 28, no. 6, pp. 612 - 620
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Brain ischemia activates Ca2+-dependent synaptic vesicle exocytosis. The synaptosomal-associated protein 25 (SNAP-25) and syntaxin proteins, located on presynaptic terminals, are components of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex and play a key role in regulating exocytosis. Changes in the expression of SNAREs could affect SNARE complex formation, fusion of vesicles with the presynaptic membrane, and release of neurotransmitters through exocytosis. To investigate the relationship of glucose/oxygen deprivation (GOD)/reperfusion-induced neuronal damage and alteration of presynaptic function, we examined the expression of SNAREs and complexin during GOD and reperfusion using organotypic hippocampal slice cultures. Microtubule-associated protein 2 (MAP-2) staining and transmission electron microscopy showed that neuronal damage increased in a time-dependent manner and both types of neuronal death can occur at different times during GOD and reperfusion. The immunoreactivity of SNAREs such as SNAP-25, vesicle-associated membrane protein and syntaxin and complexin increased in pyramidal cell bodies in the CA1 and CA3 areas in a time-dependent manner following reperfusion. Our data suggest that alteration of presynaptic function may play a partial role in delayed neuronal death during GOD and reperfusion in organotypic hippocampal slice cultures. © 2008 Japanese Society of Neuropathology.
DOI
10.1111/j.1440-1789.2008.00927.x
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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